输入关键词搜索之后,可以在左侧选择分子或者产品类型以精简过滤器,快速查找产品。如果有任何问题,请 联系我们!
Text Size:AAA

食蟹猴 PFK1/PFKM 基因全長cDNA ORF(克隆載體)

產品資料評論相關產品實驗方法
Cynomolgus PFKM cDNA Clone產品資訊
註冊碼:XM_005570698.1
cDNA基因長度:2343bp
cDNA基因描述:Full length Clone DNA of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) phosphofructokinase, muscle.
基因別名:PFKM
分子種屬:Cynomolgus
載體:pGEM-T Vector
Plasmid:pGEM-cynoPFKM
限制性酶切位點:
Tag序列:
序列資訊:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 108T/C, 912G/A not causing the amino acid variation. Please check the sequence information before order.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Product nameProduct name
研究背景

PFK1, also known as PFKM, is a regulatory glycolytic enzyme. PFK1 converts fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. It is a muscle-type isozyme. There are three phosphofructokinase isozymes in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Mutations in PFK1 gene have been related with glycogen storage disease type VII, also identified as Tarui disease.

Size / Price
貨號: CG90449-G
目錄價: 
單價:      (You Save: )
規格:
數量:+-
庫存In Stock
大包裝查詢加入購物車
Contact Us
產品圖片
      All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
      請注意:所有產品都是“僅用於科研,而不能用於診斷或治療用途”