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人 PCSK9/NARC1 基因全長cDNA ORF(克隆載體)

    產品資料評論相關產品實驗方法
     PCSK9 cDNA Clone產品資訊
    註冊碼:NM_174936.3
    cDNA基因長度:2079bp
    cDNA基因描述:Full length Clone DNA of Homo sapiens proprotein convertase subtilisin/kexin type 9.
    基因別名:FH3, PC9, NARC1, LDLCQ1, NARC-1, HCHOLA3
    分子種屬:Human
    載體:pGEM-T Vector
    Plasmid:pGEM-PCSK9
    限制性酶切位點:
    Tag序列:
    序列資訊:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1420 G/A, 2009 G/A resulting in the amino acid Val substitution by Ile, Gly substitution by Glu and 1026 A/G,1380 A/G not causing the amino acid variation.
    Sequencing primers:SP6 and T7 or M13-47 and RV-M
    ( We provide with PCSK9 qPCR primers for gene expression analysis, HP104404 )
    Promoter:
    Application:
    Antibiotic in E.coli:Ampicillin
    Antibiotic in mammalian cell:
    運輸條件:Each tube contains lyophilized plasmid.
    儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
    pGEM-T Vector Information

    The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

    pGEM-T Simple Usage Suggestion:

    The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

    Vector Sequence Download
    Product nameProduct name
    研究背景

    Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.

    References

    參考資料
  • Sseidah, N.G. et al., 2003, Proc. Natl. Acad. Sci. USA. 100: 928-933.
  • Beyer, T.P. et al., 2007, J. Lipid. Res. 48: 1488-1498
  • Shan, L. et al., 2008, Biochem. Biophys. Res. Commun. 375: 69-73.
  • Benjannet, S. et al., 2005, J. Biol. Chem. 279: 48865-48875.
  • Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.
  • Size / Price
    貨號: HG15813-G
    目錄價: 
    單價:      (You Save: )
    規格:
    數量:+-
    庫存In Stock
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    Datasheet & Documentation

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