|Recombinant Human NGF / NGFB Protein (Catalog#11050-HNAC)|
|< 3 EU/mg|
|0.2 μm filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0|
|This antibody was obtained from a rabbit immunized with purified, recombinant Human NGF / NGFB (rh NGF / NGFB; Catalog#11050-HNAC; NP_002497.2; Ser122-Arg239) and was produced using recombinant antibody technology.|
Has cross-reactivity with Mouse β-NGF (Catalog # 50385-MNAC) in ELISA assay
In a functional ELISA which immobilized recombinant Human β-NGF (Catalog # 11050-HNAC) at 2μg/mL (100 µL/well) in the plate, the Rabbit anti-Human β-NGF Monoclonal Antibody (Catalog # 11050-R711) can block the binding of 5 μg/mL of biotinylated Human NTRK1/Fc Chimera Protein (Catalog # 11073-H03H) to human β-NGF, the EC50 is 3.84 μg/mL.
The neutralization activity of antibody is Measured by its ability to neutralize βNGFinduced proliferation in the TF1 human erythroleukemic cell line. The Neutralization titer (IC50) is typically 0.0480.19 µg/mL in the presence of 10 ng/mL Recombinant Human βNGF.
Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In addition, previous studies indicated that NGF may also have an important role in the regulation of the immune system.