|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|小鼠 TCTP /TPT1 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||MG51648-ACG|
|小鼠 TCTP /TPT1 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||MG51648-ACR|
|小鼠 TCTP /TPT1 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤||MG51648-ANG|
|小鼠 TCTP /TPT1 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤||MG51648-ANR|
|小鼠 TCTP /TPT1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||MG51648-CF|
|小鼠 TCTP /TPT1 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||MG51648-CH|
|小鼠 TCTP /TPT1 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||MG51648-CM|
|小鼠 TCTP /TPT1 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||MG51648-CY|
|Mouse TPT1 Gene cDNA clone plasmid||MG51648-G|
|小鼠 TCTP /TPT1 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||MG51648-NF|
|小鼠 TCTP /TPT1 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||MG51648-NH|
|小鼠 TCTP /TPT1 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||MG51648-NM|
|小鼠 TCTP /TPT1 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||MG51648-NY|
|小鼠 TCTP /TPT1 基因全長cDNA ORF(克隆載體)||MG51648-U|
|小鼠 TCTP /TPT1 基因全長cDNA ORF克隆 (表達載體)||MG51648-UT|
Tumor protein, also known as TPT1, is a highly conserved protein among many eukaryotic organisms. Tumor protein is involved in a variety of cellular activities, including microtubule stabilization, calcium-binding activities, and apoptosis. The Mammalian translationally controlled tumour protein (TPT1) (or P23) is a protein which has been found to be preferentially synthesised in cells during the early growth phase of some types of tumour, but which is also expressed in normal cells. It was first identified as a histamine-releasing factor, acting in IgE +-dependent allergic reactions. In addition, TPT1 has been shown to bind to tubulin in the cytoskeleton, has a high affinity for calcium, is the binding target for the antimalarial compound artemisinin, and is induced in vitamin D-dependent apoptosis. TPT1 production is thought to be controlled at the translational as well as the transcriptional level.