TROY ELISA Kit, Mouse

Cat: KIT50148
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TROY ELISA Kit, Mouse General Information
Product name
TROY ELISA Kit, Mouse
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
7.88 pg/mL
Assay range
15.63-1000 pg/mL
Specificity
Recognizes both recombinant and natural Mouse TROY
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 189.16 390.60 805.08
SD 5.94 10.23 25.72
CV(%) 3.1% 2.6% 3.2%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 187.10 411.72 837.62
SD 6.24 40.28 72.45
CV(%) 3.3% 9.8% 8.6%
Recovery
The recovery of Mouse TNFRSF19 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample Average % Recovery Range
serum (n=3) 86 84 -87%
Linearity
Serum
  Recovery of detected
1:2 88%
1:4 82%
1:8 98%
1:16 102%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This TROY ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse TROY protein in Serum . It contains recombinant Mouse TROY, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
TROY ELISA Kit, Mouse Images
Mouse TNFRSF19/TROY ELISA Kit Standard Curve 128
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Mouse TNFRSF19/TROY ELISA Kit Specificity 169
This assay recognizes both recombinant and natural Mouse TNFRSF19. The factors listed above were prepared at 10 or 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
TROY ELISA Kit, Mouse Alternative Names
AL023044 ELISA Kit, Mouse;AW123854 ELISA Kit, Mouse;TAJ ELISA Kit, Mouse;TAJ-ALPHA ELISA Kit, Mouse;TRADE ELISA Kit, Mouse;Troy ELISA Kit, Mouse
TROY Background Information

Tumor necrosis factor receptor superfamily, member 19 (TNFRSF19), also known as TAJ-alpha or TROY, is a member of the TNF-receptor superfamily. TNFRSF19/TROY expression is detected in the pulmonary epithelium and the ductal epithelium of the prostate and parotid glands. TNFRSF19/TROY expression is detected in some adenocarcinoma cell lines that arise from this tissue. It has been shown to interact with TRAF family members, and to activate JNK signaling pathway when overexpressed in cells. TNFRSF19/TROY is capable of inducing apoptosis by a caspase-independent mechanism, and it is thought to play an essential role in embryonic development. TNFRSF19/TROY was negatively regulated by adipogenic transcription factor CCAAT/enhancer-binding proteins (C/EBP). TNFRSF19 signals activation of the Jnk pathway and induces cell death. Overexpression of TNFRSF19 also signals NFB activation, comparable and similar to that by p75NGFR. TNFRSF19/TROY is capable of activating key signaling pathways of the TNF receptor family, and its predominant expression patterns suggest that it plays a role in the growth and regulation of epithelial tissues.

Full Name
tumor necrosis factor receptor superfamily, member 19
References
  • Hu S, et al. (1999) Characterization of TNFRSF19, a novel member of the tumor necrosis factor receptor superfamily. Genomics. 62(1): 103-7.
  • Qiu W, et al. (2010) Tumor necrosis factor receptor superfamily member 19 (TNFRSF19) regulates differentiation fate of human mesenchymal (stromal) stem cells through canonical Wnt signaling and C/EBP. J Biol Chem. 285(19): 14438-49.
  • Hisaoka T, et al. (2006) Characterization of TROY/TNFRSF19/TAJ-expressing cells in the adult mouse forebrain. Brain Res. 1110(1): 81-94.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
    TROY Matched ELISA Antibody Pair Set,Mouse 7.8 pg/ml SEK50148
    info info
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