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小鼠 TMED4 / ERS25 基因全長cDNA ORF(克隆載體)

產品資料評論相關產品實驗方法
Mouse TMED4 cDNA Clone產品資訊
註冊碼:NM_134020.1
cDNA基因長度:684bp
cDNA基因描述:Full length Clone DNA of Mus musculus transmembrane emp24 protein transport domain containing 4.
基因別名:AI326346, 1110014L17Rik
分子種屬:Mouse
載體:PGEM-T Vector
Plasmid:pGEM-mTMED4
限制性酶切位點:
Tag序列:
序列資訊:Identical with the Gene Bank Ref. ID sequence except for the point mutation: 309A>G not causing the amino acid variation.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
Product nameProduct name
研究背景

TMED4, also known as ERS25, belongs to the EMP24/GP25L family. TMED4 may play a role in the regulation of heat-shock response and apoptosis. It is involved in vesicular protein trafficking, mainly in the early secretory pathway. TMED4 may also play a role in the biosynthesis of secreted cargo including processing. It functions in the regulation of heat-shock response and apoptosis. TMED4 also is involved in endoplasmic reticulum stress response.

參考資料
  • Hartley JL. et al., 2001, Genome Res. 10 (11): 1788-95.
  • Matoba R. et al., 1994, Gene. 146 (2): 199-207.
  • Matsuda A. et al., 2003, Oncogene. 22 (21): 3307-18.
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    貨號: MG52950-G
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