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小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤

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Mouse SRC cDNA Clone產品資訊
註冊碼:NM_009271.3
cDNA基因長度:1626bp
cDNA基因描述:Full length Clone DNA of Mus musculus Rous sarcoma oncogene transcript variant 1 with C terminal HA tag.
基因別名:AW259666, pp60c-src, Src
分子種屬:Mouse
載體:pCMV3-C-HA
Plasmid:
限制性酶切位點:
Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
序列資訊:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤 on other vectors
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤MG51118-ACGNT$8880
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤MG51118-ACRNT$8880
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤MG51118-ANGNT$8880
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤MG51118-ANRNT$8880
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤MG51118-CFNT$7790
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-His 標籤MG51118-CHNT$7790
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤MG51118-CMNT$7790
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤MG51118-CYNT$7790
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因全長cDNA ORF(克隆載體)MG51118-GNT$2720
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤MG51118-G-FNT$7790
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤MG51118-NFNT$7790
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-His 標籤MG51118-NHNT$7790
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤MG51118-NMNT$7790
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤MG51118-NYNT$7790
小鼠 SRC/Proto-oncogene c-Src transcript variant 1 基因全長cDNA ORF克隆 (表達載體)MG51118-UTNT$7790
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研究背景

Proto-oncogene tyrosine-protein kinase SRC is a hydrophobic protein belonging to the SRC family kinase including nine members that is a family of non-receptor tyrosine kinases. SRC protein may exist in different forms: C-SRC and V-SRC. C-SRC is only activated under certain circumstances where it is required such as growth factor signaling, while V-SRC is a constitutively active as opposed to normal SRC (C-SRC). Thus, V-SRC is an instructive example of an oncogene protein kinase whereas C-SRC is a proto-oncogene protein kinase. Inhibition of SRC with NR2A tyrosine phosphorylation mediated by PSD-95 may contribute to the lithium-induced downregulation of NMDA receptor function and provide neuroprotection against excitotoxicity.

參考資料
  • Juan Ma. et al., 2003, Neuroscience Letters. 348 (3): 185-189.
  • Czernilofsky AP. et al., 1980, Nature. 287: 198-203.
  • Beischlag TV. et al., 2002, Molecular and cellular biology. 22 (12): 4319-33.
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    貨號: MG51118-CY
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