|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|小鼠 OPN/Osteopontin 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||MG50116-ACG|
|小鼠 OPN/Osteopontin 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||MG50116-ACR|
|小鼠 OPN/Osteopontin 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||MG50116-CF|
|小鼠 OPN/Osteopontin 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||MG50116-CH|
|小鼠 OPN/Osteopontin 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||MG50116-CM|
|小鼠 OPN/Osteopontin 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||MG50116-CY|
|小鼠 OPN/Osteopontin 基因全長cDNA ORF(克隆載體)||MG50116-M|
|小鼠 OPN/Osteopontin 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||MG50116-NF|
|小鼠 OPN/Osteopontin 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||MG50116-NH|
|小鼠 OPN/Osteopontin 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||MG50116-NM|
|小鼠 OPN/Osteopontin 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||MG50116-NY|
|小鼠 OPN/Osteopontin 基因全長cDNA ORF克隆 (表達載體)||MG50116-UT|
Osteopontin, also known as Secreted phosphoprotein 1, Bone sialoprotein 1, BSP-1, OPN, and SPP1, is a member of the osteopontin family and a SIBLING glycoprotein. Osteopontin has been classified as T-helper 1 cytokine and thus believed to exacerbate inflammation in several chronic inflammatory diseases, including atherosclerosis. Besides proinflammatory functions, physiologically Osteopontin is a potent inhibitor of mineralization, it prevents ectopic calcium deposits and is a potent inducible inhibitor of vascular calcification. Osteopontin is expressed and secreted by various cells, and has a role in cell adhesion, chemotaxis, prevention of apoptosis, invasion, migration and anchorage-independent growth of tumor cells. Osteopontin recruitment functions of inflammatory cells are thought to be mediated through its adhesive domains, especially the arginine-glycine-aspartate (RGD) sequence that interacts with several integrin heterodimers. Osteopontin has emerged as a potential biomarker and mediator in cardiovascular disease. In the context of atherosclerosis, OPN is generally regarded as a proinflammatory and proatherogenic molecule. However, the role of OPN in vascular calcification (VC), which is closely related to chronic and active inflammation, is that of a negative regulator because it is an inhibitor of calcification and an active inducer of decalcification. Extensive research has demonstrated the pivotal participation of Osteopontin in the regulation of cell signaling which controls neoplastic and malignant transformation. The elevated expression of Osteopontin has been observed in a variety of cancers. It has been linked with tumor metastasis and signifies a poor prognosis for the patient.