|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 SerpinB6 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||MG50374-ACG|
|小鼠 SerpinB6 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||MG50374-ACR|
|小鼠 SerpinB6 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤||MG50374-ANG|
|小鼠 SerpinB6 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤||MG50374-ANR|
|小鼠 SerpinB6 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||MG50374-CF|
|小鼠 SerpinB6 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||MG50374-CH|
|小鼠 SerpinB6 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||MG50374-CM|
|小鼠 SerpinB6 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||MG50374-CY|
|小鼠 SerpinB6 基因全長cDNA ORF(克隆載體)||MG50374-M|
|小鼠 SerpinB6 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||MG50374-NF|
|小鼠 SerpinB6 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||MG50374-NH|
|小鼠 SerpinB6 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||MG50374-NM|
|小鼠 SerpinB6 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||MG50374-NY|
|小鼠 SerpinB6 基因全長cDNA ORF克隆 (表達載體)||MG50374-UT|
SerpinB6, also known as Cytoplasmic antiproteinase, Peptidase inhibitor 6, Placental thrombin inhibitor, SERPINB6 and PI-6, is a cytoplasm protein which belongs to the serpin family and Ov-serpin subfamily. SerpinB6 / PI-6 is an inhibitor of cathepsin G, kallikrein-8 and thrombin. It may play an important role in the inner ear in the protection against leakage of lysosomal content during stress and loss of this protection results in cell death and sensorineural hearing loss. SerpinB6 / PI-6 is expressed in keratinocytes (at protein level). It is also found in placenta, cardiac muscle, lung, liver, kidney and pancreas. SerpinB6 / PI-6 is expressed in the inner ear hair cells. It expressed abundantly by normal mast cells in different tissues and by mast cells in mastocytoma lesions. SerpinB6 / PI-6 may be involved in the regulation of serine proteinases present in the brain or extravasated from the blood. Defects in SerpinB6 are the cause of deafness autosomal recessive type 91 which is a form of non-syndromic deafness characterized by progressive and age-dependent sensorineural hearing loss. Vestibular function is normal.