( We provide with S100A13 qPCR primers for gene expression analysis, MP200235 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 S100A13 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||MG50207-ACG|
|小鼠 S100A13 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||MG50207-ACR|
|小鼠 S100A13 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤||MG50207-ANG|
|小鼠 S100A13 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤||MG50207-ANR|
|小鼠 S100A13 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||MG50207-CF|
|小鼠 S100A13 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||MG50207-CH|
|小鼠 S100A13 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||MG50207-CM|
|小鼠 S100A13 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||MG50207-CY|
|小鼠 S100A13 基因全長cDNA ORF(克隆載體)||MG50207-M|
|小鼠 S100A13 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||MG50207-NF|
|小鼠 S100A13 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||MG50207-NH|
|小鼠 S100A13 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||MG50207-NM|
|小鼠 S100A13 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||MG50207-NY|
|小鼠 S100A13 基因全長cDNA ORF克隆 (表達載體)||MG50207-UT|
S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. Protein S100-A13, also known as S100 calcium-binding protein A13, is a member of the S-100 family. It contains two EF-hand domains. S100A13 binds two calcium ions per subunit and one copper ion. Binding of one copper ion does not interfere with calcium binding. S100A13 is required for the copper-dependent stress-induced export of IL1A and FGF1. The calcium-free protein binds to lipid vesicles containing phosphatidylserine, but not to vesicles containing phosphatidylcholine. S100A13 plays a role in the export of proteins that lack a signal peptide and are secreted by an alternative pathway.