|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 PTPN2/TC-PTP 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||MG50800-ACG|
|小鼠 PTPN2/TC-PTP 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||MG50800-ACR|
|小鼠 PTPN2/TC-PTP 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||MG50800-CF|
|小鼠 PTPN2/TC-PTP 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||MG50800-CH|
|小鼠 PTPN2/TC-PTP 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||MG50800-CM|
|小鼠 PTPN2/TC-PTP 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||MG50800-CY|
|小鼠 PTPN2/TC-PTP 基因全長cDNA ORF(克隆載體)||MG50800-G|
|小鼠 PTPN2/TC-PTP 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||MG50800-NF|
|小鼠 PTPN2/TC-PTP 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||MG50800-NH|
|小鼠 PTPN2/TC-PTP 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||MG50800-NM|
|小鼠 PTPN2/TC-PTP 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||MG50800-NY|
|小鼠 PTPN2/TC-PTP 基因全長cDNA ORF克隆 (表達載體)||MG50800-UT|
Tyrosine-protein phosphatase non-receptor type 2, also known as T-cell protein-tyrosine phosphatase, PTPN2 and PTPT, is a cytoplasm protein which belongs to the protein-tyrosine phosphatase family and Non-receptor class 1 subfamily. Members of the protein tyrosine phosphatase ( PTP ) family share a highly conserved catalytic motif, which is essential for the catalytic activity. TC-PTP / PTPN2 is a cytosolic tyrosine phosphatase that functions as a negative regulator of a variety of tyrosine kinases and other signaling proteins. The expression of TC-PTP / PTPN2 plays a role of tumor suppressor and may modulate response to treatment. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. TC-PTP / PTPN2 is an enzyme that is essential for the proper functioning of the immune system and that participates in the control of cell proliferation, and inflammation. TC-PTP / PTPN2 was identified as a negative regulator of NUP214-ABL1 kinase activity.