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小鼠 PSMA/FOLH1/GCPII 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤

  • Mouse PSMA / FOLH1 ORF mammalian expression plasmid, N-Myc tag
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小鼠 FOLH1 cDNA Clone產品資訊
註冊碼:NM_016770.2
cDNA基因長度:2259bp
cDNA基因描述:Full length Clone DNA of Mus musculus folate hydrolase with N terminal Myc tag.
基因別名:mopsm, MGC141397, Folh1
分子種屬:Mouse
載體:pCMV3-SP-N-Myc
Plasmid:pCMV3-Myc-mFOLH1
限制性酶切位點:HindIII + XbaI (6kb + 2.35kb)
Tag序列:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
序列資訊:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1842G/A, 2345C/T, 2347T/A not causing the amino acid variation.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with FOLH1 qPCR primers for gene expression analysis, MP200242 )
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
小鼠 FOLH1 Gene Plasmid Map
Mouse PSMA / FOLH1 ORF mammalian expression plasmid, N-Myc tag
Myc Tag Info

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

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研究背景

Glutamate carboxypeptidase 2, also known as Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, Prostate-specific membrane antigen, GCPII, PSMA, FOLH1, and NAALAD1, is a single-pass type I I membrane protein which belongs to the peptidase M28 family and M28B subfamily. FOLH1 is highly expressed in prostate epithelium. It is detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon, brain (at protein level), and the capillary endothelium of a variety of tumors. FOLH1 has both folate hydrolase and N-acetylated alpha linked acidic dipeptidase (NAALADase) activity. It has a preference for tri-alpha-glutamate peptides. Genetic variation in FOLH1 may be associated with low folate levels and consequent hyperhomocysteinemia. This condition can result in increased risk of cardiovascular disease, neural tube defects, and cognitive deficits. FOLH1 also shows a promising role in directed imaging and therapy of recurrent or metastatic disease.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

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貨號: MG50214-NM
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