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小鼠 PFK1/PFKM 基因全長cDNA ORF克隆 (表達載體), N-His 標籤

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    小鼠 PFKM cDNA Clone產品資訊
    註冊碼:NM_001163487.1
    cDNA基因長度:2343bp
    cDNA基因描述:Full length Clone DNA of Mus musculus phosphofructokinase, muscle with N terminal His tag.
    基因別名:Pfk4, Pfka, Pfkx, PFK-A, PFK-M, Pfk-4, AI131669
    分子種屬:Mouse
    載體:pCMV3-N-His
    Plasmid:
    限制性酶切位點:
    Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
    序列資訊:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with PFKM qPCR primers for gene expression analysis, MP202414 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    運輸條件:Each tube contains lyophilized plasmid.
    儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
    His Tag Info

    A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

    Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

    Product nameProduct name
    研究背景

    PFK1, also known as PFKM, is a regulatory glycolytic enzyme. PFK1 converts fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. It is a muscle-type isozyme. There are three phosphofructokinase isozymes in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Mutations in PFK1 gene have been related with glycogen storage disease type VII, also identified as Tarui disease.

    Size / Price
    貨號: MG52541-NH
    目錄價: 
    單價:      (You Save: )

    Datasheet & Documentation

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