|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|小鼠 Osteomodulin 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||MG50451-ACG|
|小鼠 Osteomodulin 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||MG50451-ACR|
|小鼠 Osteomodulin 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||MG50451-CF|
|小鼠 Osteomodulin 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||MG50451-CH|
|小鼠 Osteomodulin 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||MG50451-CM|
|小鼠 Osteomodulin 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||MG50451-CY|
|小鼠 Osteomodulin 基因全長cDNA ORF(克隆載體)||MG50451-M|
|小鼠 Osteomodulin 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||MG50451-NF|
|小鼠 Osteomodulin 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||MG50451-NH|
|小鼠 Osteomodulin 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||MG50451-NM|
|小鼠 Osteomodulin 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||MG50451-NY|
|小鼠 Osteomodulin 基因全長cDNA ORF克隆 (表達載體)||MG50451-UT|
Osteomodulin (OMD), also known as Osteoadherin (OSAD), Keratan sulfate proteoglycan osteomodulin, KSPG osteomodulin, and SLRR2C, is a secreted protein which belongs to the small leucine-rich proteoglycan (SLRP) family and Class II subfamily. SLRP family proteins are normally found in extracellular matrices, but Osteomodulin is the only member restricted to mineralized tissues. Osteomodulin is primarily expressed by osteoblasts and might have a role in regulation of mineralization. In bone OSAD has been localized in primary spongiosa within the bovine fetal rib growth plate. Moreover, in situ hybridization has shown expression of OSAD in osteoblasts close to the cartilage and bone border in the growth plate of rat femur. OSAD may play an important role during tooth development and biomineralization of dentin. Osteomodulin is a cell binding keratan sulfate proteoglycan which was recently isolated from mineralized bovine bone and subsequently cloned and sequenced. Osteomodulin may be implicated in biomineralization processes. It has a function in binding of osteoblasts via the alpha (V) beta (3)-integrin. It is likely that Osteomodulin is an osteoblast maturation marker that is induced by osteoclast activity. Osteomodulin is also an early marker for terminally differentiated matrix producing osteoblasts.