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小鼠 MDM2 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤

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    小鼠 MDM2 cDNA Clone產品資訊
    註冊碼:NM_010786.3
    cDNA基因長度:1470bp
    cDNA基因描述:Full length Clone DNA of Mus musculus transformed mouse 3T3 cell double minute 2 with N terminal HA tag.
    基因別名:Mdm-2, AA415488, 1700007J15Rik, Mdm2
    分子種屬:Mouse
    載體:pCMV3-N-HA
    Plasmid:
    限制性酶切位點:
    Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
    序列資訊:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with MDM2 qPCR primers for gene expression analysis, MP200525 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    運輸條件:Each tube contains lyophilized plasmid.
    儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
    HA Tag Info

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

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    研究背景

    MDM2 gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.

    Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

    參考資料
  • Zhuo X, Ye H, Li Q, Xiang Z, Zhang X. Is MDM2 SNP309 Variation a Risk Factor for Head and Neck Carcinoma?: An Updated Meta-Analysis Based on 11,552 Individuals. Langevin. S, ed. Medicine. 2016;95(9):e2948.
  • Size / Price
    貨號: MG50533-NY
    目錄價: 
    單價:      (You Save: )

    Datasheet & Documentation

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