IL-16 ELISA Kit, Mouse

Cat: KIT51303

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IL-16 ELISA Kit, Mouse General Information
Product name
IL-16 ELISA Kit, Mouse
Assay type
Solid Phase Sandwich ELISA (quantitative)
Detection antibody conjugate
Sample type
Serum,Cell supernatant
2.97 pg/mL
Assay range
5.17-350 pg/mL
Recognizes both recombinant and natural Mouse IL-16
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 38.00 70.00 144.00
SD 1.13 1.69 5.13
CV(%) 3.0% 2.4% 3.6%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 36.00 75.00 148.00
SD 3.05 2.95 10.50
CV(%) 8.4% 3.9% 7.1%
The recovery of Mouse IL16 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample Average % Recovery Range
serum (n=3) 81 78 -86%
supernatant (n=3) 122 115 -126%
urine (n=3) 84 81 -87%
  Recovery of detected
1:2 71%
1:4 85%
1:8 115%
  Recovery of detected
1:2 128%
1:4 106%
1:8 95%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This IL-16 ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-16 protein in Serum,Cell supernatant . It contains recombinant Mouse IL-16, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
This ELISA Kit is shipped at ambient temperature.
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
IL-16 ELISA Kit, Mouse Images
Mouse IL16 ELISA Kit Standard Curve 139
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Mouse IL16 ELISA Kit Specificity 180
This assay recognizes both recombinant and natural Mouse IL16. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.Cross-reactivity was observed to be 0.46% with 50 ng/mL of recombinant human IL16.
IL-16 ELISA Kit, Mouse Alternative Names
mKIAA4048 ELISA Kit, Mouse
IL-16 Background Information
Full Name
interleukin 16
  • Maciaszek JW, et al. (1997) IL-16 represses HIV-1 promoter activity. J Immunol. 158(1): 5-8.
  • Zhou P, et al. (1997) Human CD4+ cells transfected with IL-16 cDNA are resistant to HIV-1 infection: inhibition of mRNA expression. Nat Med. 3(6): 659-64.
  • Theodore AC, et al. (1996) CD4 ligand IL-16 inhibits the mixed lymphocyte reaction. J Immunol. 157(5): 1958-64.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
    IL-16 Matched ELISA Antibody Pair Set,Mouse 3.13 pg/ml SEK51303
    IL-16 Matched ELISA Antibody Pair Set,Human 9.4 pg/ml SEK10944
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