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小鼠 IL-18 ELISA Kit

  • Mouse IL-18 ELISA Kit, TYPICAL DATA
  • Mouse IL-18 ELISA Kit, SPECIFICITY
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檢測原理
Solid Phase Sandwich ELISA
預期用途
For the quantitative determination of Mouse IL-18 concentration in serum.
The use of this kit for other sample types need be validated by the end user due to the complexity of natural targets and unpredictable interference.
線性範圍
23.44-1500 pg/mL
靈敏度
The minimum detectable dose of Mouse IL-18 is typically less than 21.03 pg/mL. The MDD was determined by adding three standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
試劑盒組分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
儲存方法
小鼠 IL18 ELISA Kit Standard Curve
Mouse IL-18 ELISA Kit, TYPICAL DATA
[點擊查看大圖]
Caption:
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
小鼠 IL18 ELISA Kit Specificity
Mouse IL-18 ELISA Kit, SPECIFICITY
[點擊查看大圖]
Caption:
This assay recognizes both recombinant and natural Mouse IL18. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
IL18/IL-18/Interleukin 18 研究背景

Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1−like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cells-, IL-4− and STAT6−dependent fashion. IL-18 and T cell receptor−mediated stimulation could induce naïve CD4+ T cells to develop into IL-4−producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

小鼠 IL18/IL-18/Interleukin 18 參考資料
  • Dinarello CA. (1999) IL-18: A TH1-inducing, proinflammatory cytokine and new member of the IL-1 family. J Allergy Clin Immunol. 103: 11-24.
  • Takeda K, et al.. (1998) Defective NK cell activity and Th1 response in IL-18-deficient mice. Immunity. 8(3): 383-90.
  • Gracie JA, et al.. (1999) A proinflammatory role for IL-18 in rheumatoid arthritis. J Clin Invest. 104(10): 1393-401.
  • Size / Price
    貨號: KIT50073-1
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    Datasheet & Documentation

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