Product name
IL-18 ELISA Kit, Mouse
Assay type
Solid Phase Sandwich ELISA (quantitative)
Assay range
23.44-1500 pg/mL
Specificity
Recognizes both recombinant and natural Mouse IL-18
Interference
Preparations of Mouse IL18R1 at 15 ng/mL in a mid-range recombinant Mouse IL18 control were assayed for interference. No significant interference was observed.
Precision
| |
Intra-assay Precision |
| Sample |
1 |
2 |
3 |
| N |
20 |
20 |
20 |
| Mean(pg/mL) |
332.00 |
558.00 |
1,013.00 |
| SD |
10.48 |
15.59 |
36.90 |
| CV(%) |
3.2% |
2.8% |
3.6% |
| |
Inter-assay Precision |
| Sample |
1 |
2 |
3 |
| N |
20 |
20 |
20 |
| Mean(pg/mL) |
221.00 |
448.00 |
884.00 |
| SD |
6.95 |
3.83 |
34.30 |
| CV(%) |
3.2% |
0.9% |
3.9% |
Recovery
The recovery of Mouse IL18 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
Average % Recovery |
Range |
| serum (n=3) |
73.6360624355765 |
72%-75% |
Linearity
Serum
| |
Recovery of detected |
| 1:2 |
108% |
| 1:4 |
121% |
| 1:8 |
124% |
| 1:16 |
108% |
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This IL-18 ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-18 protein in Serum . It contains recombinant Mouse IL-18, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1−like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cells-, IL-4− and STAT6−dependent fashion. IL-18 and T cell receptor−mediated stimulation could induce naïve CD4+ T cells to develop into IL-4−producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.
Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
References
Dinarello CA. (1999) IL-18: A TH1-inducing, proinflammatory cytokine and new member of the IL-1 family. J Allergy Clin Immunol. 103: 11-24. Takeda K, et al.. (1998) Defective NK cell activity and Th1 response in IL-18-deficient mice. Immunity. 8(3): 383-90. Gracie JA, et al.. (1999) A proinflammatory role for IL-18 in rheumatoid arthritis. J Clin Invest. 104(10): 1393-401.
