IL-18 ELISA Kit, Mouse

Cat: KIT50073
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IL-18 ELISA Kit, Mouse General Information
Product name
IL-18 ELISA Kit, Mouse
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
21.03 pg/mL
Assay range
23.44-1500 pg/mL
Specificity
Recognizes both recombinant and natural Mouse IL-18
Interference
Preparations of Mouse IL18R1 at 15 ng/mL in a mid-range recombinant Mouse IL18 control were assayed for interference. No significant interference was observed.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 332.00 558.00 1,013.00
SD 10.48 15.59 36.90
CV(%) 3.2% 2.8% 3.6%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 221.00 448.00 884.00
SD 6.95 3.83 34.30
CV(%) 3.2% 0.9% 3.9%
Recovery
The recovery of Mouse IL18 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample Average % Recovery Range
serum (n=3) 73.6360624355765 72%-75%
Linearity
Serum
  Recovery of detected
1:2 108%
1:4 121%
1:8 124%
1:16 108%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This IL-18 ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-18 protein in Serum . It contains recombinant Mouse IL-18, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
IL-18 ELISA Kit, Mouse Images
Mouse IL18/IL-18/Interleukin 18 ELISA Kit Standard Curve 123
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Mouse IL18/IL-18/Interleukin 18 ELISA Kit Specificity 179
This assay recognizes both recombinant and natural Mouse IL18. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
IL-18 ELISA Kit, Mouse Alternative Names
Igif ELISA Kit, Mouse;Il-18 ELISA Kit, Mouse
IL-18 Background Information

Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1−like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cells-, IL-4− and STAT6−dependent fashion. IL-18 and T cell receptor−mediated stimulation could induce naïve CD4+ T cells to develop into IL-4−producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
interleukin 18
References
  • Dinarello CA. (1999) IL-18: A TH1-inducing, proinflammatory cytokine and new member of the IL-1 family. J Allergy Clin Immunol. 103: 11-24.
  • Takeda K, et al.. (1998) Defective NK cell activity and Th1 response in IL-18-deficient mice. Immunity. 8(3): 383-90.
  • Gracie JA, et al.. (1999) A proinflammatory role for IL-18 in rheumatoid arthritis. J Clin Invest. 104(10): 1393-401.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
    IL-18 Matched ELISA Antibody Pair Set,Mouse 23.44 pg/ml SEK50073
    IL-18 Matched ELISA Antibody Pair Set,Human 31.25 pg/ml SEK10119
    IL-18 ELISA Kit, Human Serum KIT10119
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