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小鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF(克隆載體)

產品資料評論相關產品實驗方法
Mouse GLRX cDNA Clone產品資訊
註冊碼:NM_053108.4
cDNA基因長度:324bp
cDNA基因描述:Full length Clone DNA of Mus musculus glutaredoxin.
基因別名:Grx1, Glrx1, TTase, C86710, D13Wsu156e
分子種屬:Mouse
載體:PGEM-T Vector
Plasmid:pGEM-mGLRX
限制性酶切位點:
Tag序列:
序列資訊:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
小鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF(克隆載體) on other vectors
小鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤MG52947-ACGNT$8150
小鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤MG52947-ACRNT$8150
小鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤MG52947-ANGNT$8150
小鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤MG52947-ANRNT$8150
小鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤MG52947-CFNT$7060
小鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), C-His 標籤MG52947-CHNT$7060
小鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤MG52947-CMNT$7060
小鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤MG52947-CYNT$7060
小鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF(克隆載體)MG52947-GNT$2720
小鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤MG52947-NFNT$7060
小鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), N-His 標籤MG52947-NHNT$7060
小鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤MG52947-NMNT$7060
小鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤MG52947-NYNT$7060
小鼠 glutaredoxin-1 / GRX1 / GLRX 基因全長cDNA ORF克隆 (表達載體)MG52947-UTNT$7060
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研究背景

Glutaredoxin-1, also known as GRX1 and GLRX, belongs to the glutaredoxin family. Glutaredoxins are small redox enzymes that use glutathione as a cofactor. Glutaredoxins are oxidized by substrates, and reduced non-enzymatically by glutathione. Glutaredoxin-1 functions as an electron carrier in the glutathione-dependent synthesis of deoxyribonucleotides by the enzyme ribonucleotide reductase. Glutaredoxin-1 exists in either a reduced or an oxidized form. Glutaredoxins function as electron carriers in the glutathione-dependent synthesis of deoxyribonucleotides by the enzymeribonucleotide reductase.

參考資料
  • Holmgren A. et al., 1988, FEMS Microbiol Rev. 4 (4): 271-97.
  • Holmgren A. 1988, Biochem Soc Trans. 16 (2): 95-6.
  • Holmgren A. 1989, J Biol Chem. 264 (24): 13963-6.
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