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小鼠 UDP galactose-4'-epimerase / GALE 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤

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    小鼠 GALE cDNA Clone產品資訊
    註冊碼:NM_178389.3
    cDNA基因長度:1044bp
    cDNA基因描述:Full length Clone DNA of Mus musculus galactose-4-epimerase, UDP with C terminal HA tag.
    基因別名:AI323962, 2310002A12Rik
    分子種屬:Mouse
    載體:pCMV3-C-HA
    Plasmid:
    限制性酶切位點:
    Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
    序列資訊:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with GALE qPCR primers for gene expression analysis, MP201643 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    運輸條件:Each tube contains lyophilized plasmid.
    儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
    HA Tag Info

    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

    小鼠 UDP galactose-4'-epimerase / GALE 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤 on other vectors
    小鼠 UDP galactose-4'-epimerase / GALE 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤MG51770-ACGNT$8150
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    小鼠 UDP galactose-4'-epimerase / GALE 基因全長cDNA ORF克隆 (表達載體), C-His 標籤MG51770-CHNT$7060
    小鼠 UDP galactose-4'-epimerase / GALE 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤MG51770-CMNT$7060
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    小鼠 UDP galactose-4'-epimerase / GALE 基因全長cDNA ORF(克隆載體)MG51770-GNT$2720
    小鼠 UDP galactose-4'-epimerase / GALE 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤MG51770-NFNT$7060
    小鼠 UDP galactose-4'-epimerase / GALE 基因全長cDNA ORF克隆 (表達載體), N-His 標籤MG51770-NHNT$7060
    小鼠 UDP galactose-4'-epimerase / GALE 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤MG51770-NMNT$7060
    小鼠 UDP galactose-4'-epimerase / GALE 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤MG51770-NYNT$7060
    小鼠 UDP galactose-4'-epimerase / GALE 基因全長cDNA ORF克隆 (表達載體)MG51770-UTNT$7060
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    研究背景

    UDP galactose-4'-epimerase, also known as GALE, enables the body to process a simple sugar called galactose, which is present in small amounts in many foods. Galactose is primarily part of a larger sugar called lactose, which is found in all dairy products and many baby formulas. UDP galactose-4'-epimerase catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. Defects in GALE causes epimerase-deficiency galactosemia (EDG), also known as galactosemia type 3. Clinical features include early-onset cataracts, liver damage, deafness and mental retardation.

    參考資料
  • Kim W. et al., 2011, Mol Cell. 44 (2): 325-40.
  • Lee KA. et al., 2011,. J Biol Chem. 286 (48): 41530-8.
  • McCorvie TJ. et al., 2012, Biochim Biophys Acta. 1822 (10): 1516-26.
  • Size / Price
    貨號: MG51770-CY
    目錄價: 
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