|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 FGFR4/CD334 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||MG50194-ACG|
|小鼠 FGFR4/CD334 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||MG50194-ACR|
|小鼠 FGFR4/CD334 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||MG50194-CF|
|小鼠 FGFR4/CD334 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||MG50194-CH|
|小鼠 FGFR4/CD334 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||MG50194-CM|
|小鼠 FGFR4/CD334 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||MG50194-CY|
|小鼠 FGFR4/CD334 基因全長cDNA ORF(克隆載體)||MG50194-M|
|小鼠 FGFR4/CD334 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||MG50194-NF|
|小鼠 FGFR4/CD334 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||MG50194-NH|
|小鼠 FGFR4/CD334 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||MG50194-NM|
|小鼠 FGFR4/CD334 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||MG50194-NY|
|小鼠 FGFR4/CD334 基因全長cDNA ORF克隆 (表達載體)||MG50194-UT|
Fibroblast growth factor receptor 4 (FGFR4) also known as CD334 antigen or tyrosine kinase related to fibroblast growth factor receptor, is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of FGFR4/CD334 interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. FGFR4/CD334 preferentially binds acidic fibroblast growth factor and, although its specific function is unknown, it is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis. FGFR4/CD334 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4/CD334. Mutations in FGFR4/CD334 lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling.