|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|小鼠 ERK2/MAPK1/MAPK2 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||MG50445-ACG|
|小鼠 ERK2/MAPK1/MAPK2 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||MG50445-ACR|
|小鼠 ERK2/MAPK1/MAPK2 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤||MG50445-ANG|
|小鼠 ERK2/MAPK1/MAPK2 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤||MG50445-ANR|
|小鼠 ERK2/MAPK1/MAPK2 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||MG50445-CF|
|小鼠 ERK2/MAPK1/MAPK2 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||MG50445-CH|
|小鼠 ERK2/MAPK1/MAPK2 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||MG50445-CM|
|小鼠 ERK2/MAPK1/MAPK2 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||MG50445-CY|
|小鼠 ERK2/MAPK1/MAPK2 基因全長cDNA ORF(克隆載體)||MG50445-G|
|小鼠 ERK2/MAPK1/MAPK2 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||MG50445-NF|
|小鼠 ERK2/MAPK1/MAPK2 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||MG50445-NH|
|小鼠 ERK2/MAPK1/MAPK2 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||MG50445-NM|
|小鼠 ERK2/MAPK1/MAPK2 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||MG50445-NY|
|小鼠 ERK2/MAPK1/MAPK2 基因全長cDNA ORF克隆 (表達載體)||MG50445-UT|
MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. ERK is a versatile protein kinase that regulates many cellular functions. Growing evidence suggests that extracellular signal-regulated protein kinase 1/2 (ERK1/2) plays a crucial role in promoting cell death in a variety of neuronal systems, including neurodegenerative diseases. It is believed that the magnitude and the duration of ERK1/2 activity determine its cellular function. Activation of ERK1/2 are implicated in the pathophysiology of spinal cord injury (SCI). ERK2 signaling is a novel target associated with the deleterious consequences of spinal injury. ERK-2, also known as Mitogen-activated protein kinase 1 (MAPK1), is a member of the protein kinase superfamily and MAP kinase subfamily. MKP-3 is a dual specificity phosphatase exclusively specific to MAPK1 for its substrate recognition and dephosphorylating activity. The activation of MAPK1 requires its phosphorylation by upstream kinases. Upon activation, MAPK1 translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. MAPK1 is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. MAPK1 acts as a transcriptional repressor which represses the expression of interferon gamma-induced genes. Transcriptional activity is independent of kinase activity. The nuclear-cytoplasmic distribution of ERK2 is regulated in response to various stimuli and changes in cell context. Furthermore, the nuclear flux of ERK2 occurs by several energy- and carrier-dependent and -independent mechanisms. ERK2 has been shown to translocate into and out of the nucleus by facilitated diffusion through the nuclear pore, interacting directly with proteins within the nuclear pore complex, as well as by karyopherin-mediated transport. ERK2 interacts with the PDE4 catalytic unit by binding to a KIM (kinase interaction motif) docking site located on an exposed beta-hairpin loop and an FQF (Phe-Gln-Phe) specificity site located on an exposed alpha-helix. These flank a site that allows phosphorylation by ERK, the functional outcome of which is orchestrated by the N-terminal UCR1/2 (upstream conserved region 1 and 2) modules.