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小鼠 DKK3 ELISA Kit

  • Mouse DKK3 ELISA Kit, TYPICAL DATA
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檢測原理
Solid Phase Sandwich ELISA
預期用途
For the quantitative determination of Mouse DKK3 concentration in serum.
The use of this kit for other sample types need be validated by the end user due to the complexity of natural targets and unpredictable interference.
線性範圍
12.5-800 pg/mL
靈敏度
The minimum detectable dose of Mouse DKK3 is typically less than 4.62 pg/mL. The MDD was determined by adding three standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
試劑盒組分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
儲存方法
小鼠 DKK3 ELISA Kit Standard Curve
Mouse DKK3 ELISA Kit, TYPICAL DATA
[點擊查看大圖]
Caption:
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Coating: Coat corresponding Protein (20 μg/mL in pH 9.6 carbonate buffer) in NUNC plate
Sample: Add the indicated amounts of M13 Bacteriophages displaying Rabbit/Mouse Fab targeted for different proteins;
Primary Antibody: M13 Antibody (11973-MM05T), 0.365 μg/mL
Secondary Antibody: anti-mouse F(ab)2 Antibody/HRP
DKK3 研究背景

DKK3 (dickkopf related protein 3) is a member of the dickkopf-related family consisting of DKK1, DKK2, DKK3 and DKK4. It is a secreted protein, and also known as REIC (Reduced Expansion in Immortalized Cells). The DKK3 protein is proposed to function as a secreted tumor suppressor since it is downregulated in a number of cancer cells and prostate cancer tissue and may be a promising candidate molecule for therapeutic interference. DKK3 protein is also a negative regulator of beta-catenin and its downregulation contribute to an activation of the beta-catenin signaling pathway.

小鼠 DKK3 參考資料
  • Yue W, et al., 2008. Downregulation of Dkk3 activates beta-catenin/TCF-4 signaling in lung cancer. Carcinogenesis, 29 (1): 84-92.
  • Ding Z, et al., 2009. Promoter methylation and mRNA expression of DKK3 and WIF-1 in hepatocellular carcinoma. World J Gastroenterol, 15 (21):2595-601.
  • Size / Price
    貨號: KIT50247-1
    目錄價: 
    單價:      (You Save: )

    Datasheet & Documentation

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