DKK3 ELISA Kit, Mouse

Cat: KIT50247
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DKK3 ELISA Kit, Mouse General Information
Product name
DKK3 ELISA Kit, Mouse
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
4.62 pg/mL
Assay range
12.5-800 pg/mL
Specificity
Recognizes both recombinant and natural Mouse DKK3
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 132.00 243.00 481.00
SD 7.98 7.82 12.03
CV(%) 6.0% 3.2% 2.5%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 132.00 262.00 495.00
SD 4.51 19.00 29.02
CV(%) 3.4% 7.3% 5.9%
Recovery
The recovery of Mouse DKK3 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample Average % Recovery Range
serum (n=3) 83 82 -84%
Linearity
Serum
  Recovery of detected
1:2 95%
1:4 103%
1:8 99%
1:16 101%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This DKK3 ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse DKK3 protein in Serum . It contains recombinant Mouse DKK3, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
DKK3 ELISA Kit, Mouse Images
Mouse DKK3 ELISA Kit Standard Curve 131
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
DKK3 ELISA Kit, Mouse Alternative Names
AW061014 ELISA Kit, Mouse;C87148 ELISA Kit, Mouse
DKK3 Background Information

DKK3 (dickkopf related protein 3) is a member of the dickkopf-related family consisting of DKK1, DKK2, DKK3 and DKK4. It is a secreted protein, and also known as REIC (Reduced Expansion in Immortalized Cells). The DKK3 protein is proposed to function as a secreted tumor suppressor since it is downregulated in a number of cancer cells and prostate cancer tissue and may be a promising candidate molecule for therapeutic interference. DKK3 protein is also a negative regulator of beta-catenin and its downregulation contribute to an activation of the beta-catenin signaling pathway.

Full Name
dickkopf WNT signaling pathway inhibitor 3
References
  • Yue W, et al., 2008. Downregulation of Dkk3 activates beta-catenin/TCF-4 signaling in lung cancer. Carcinogenesis, 29 (1): 84-92.
  • Ding Z, et al., 2009. Promoter methylation and mRNA expression of DKK3 and WIF-1 in hepatocellular carcinoma. World J Gastroenterol, 15 (21):2595-601.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
    DKK3 Matched ELISA Antibody Pair Set,Mouse 15.625 pg/ml SEK50247
    DKK3 Matched ELISA Antibody Pair Set,Human 39.1 pg/ml SEKA10203
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