|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 Cathepsin A/CTSA 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||MG50348-ACG|
|小鼠 Cathepsin A/CTSA 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||MG50348-ACR|
|小鼠 Cathepsin A/CTSA 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||MG50348-CF|
|小鼠 Cathepsin A/CTSA 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||MG50348-CH|
|小鼠 Cathepsin A/CTSA 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||MG50348-CM|
|小鼠 Cathepsin A/CTSA 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||MG50348-CY|
|小鼠 Cathepsin A/CTSA 基因全長cDNA ORF(克隆載體)||MG50348-M|
|小鼠 Cathepsin A/CTSA 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||MG50348-NF|
|小鼠 Cathepsin A/CTSA 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||MG50348-NH|
|小鼠 Cathepsin A/CTSA 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||MG50348-NM|
|小鼠 Cathepsin A/CTSA 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||MG50348-NY|
|小鼠 Cathepsin A/CTSA 基因全長cDNA ORF克隆 (表達載體)||MG50348-UT|
Lysosomal carboxypeptidase, cathepsin A (protective protein, CathA), is a component of the lysosomal multienzyme complex along with beta-galactosidase (GAL) and sialidase Neu1, where it activates Neu1 and protects GAL and Neu1 against the rapid proteolytic degradation. Cathepsin A is a multicatalytic enzyme with deamidase and esterase in addition to carboxypeptidase activities. It was recently identified in human platelets as deamidase. In vitro, it hydrolyzes a variety of bioactive peptide hormones including tachykinins, suggesting that extralysosomal cathepsin A plays a role in regulation of bioactive peptide functions. It is a member of the alpha/beta hydrolase fold family and has been suggested to share a common ancestral relationship with other alpha/beta hydrolase fold enzymes, such as cholinesterases. Cathepsin A defects are linked to multiple forms of Galactosialidosis with a combined secondary deficiency of beta-galactosidase and neuraminidase. Cathepsin A is a key molecule in the onset of galactosialidosis and also highlight the therapeutic acts in vivo as an endothelin-1-inactivating enzyme and strongly confirm a crucial role of this enzyme in effective elastic fiber formation.