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小鼠 CKM / CK-MM 基因全長cDNA ORF克隆 (表達載體)

  • other Green fluorescent protein / GFP Gene Plasmid Map 5613
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小鼠 CKM cDNA Clone產品資訊
註冊碼:X03233.1
cDNA基因長度:1146bp
cDNA基因描述:Full length Clone DNA of Mus musculus creatine kinase, muscle.
基因別名:MCK, Ckmm, M-CK
分子種屬:Mouse
載體:pCMV3-untagged
Plasmid:pCMV3-mCKM
限制性酶切位點:KpnI + XbaI(6.1kb+1.15kb)
Tag序列:
序列資訊:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 147C/T not causing the amino acid variation.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with CKM qPCR primers for gene expression analysis, MP201233 )
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Ampicillin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
Product nameProduct name
研究背景

CKM, also known as CK-MM, is mainly expressed in skeletal muscle. As the primary CK isoenzyme, it also can be detected in heart muscle. CKM is a subunit of creatine kinase (CK). CK is an enzyme expressed by various tissues and cell types. It catalyses the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine and adenosine diphosphate (ADP). In the cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type).

參考資料
  • Goldblatt H. 1969, Laboratory Investigation. 21 (2): 126-8.
  • Hekimsoy Zeliha. et al., 2005, Endocrine Research. 31 (3): 171-5.
  • Döring F. et al., 2011, Scand J Med Sci Sports. 21(6):841-5.
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    貨號: MG51356-UT
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