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小鼠 CKB / Creatine kinase B type 基因全長cDNA ORF克隆 (表達載體)

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Mouse CKB cDNA Clone產品資訊
註冊碼:NM_021273.4
cDNA基因長度:1146bp
cDNA基因描述:Full length Clone DNA of Mus musculus creatine kinase, brain.
基因別名:Bck, Ck3, B-CK, Ck-3, Ckbb
分子種屬:Mouse
載體:pCMV3-untagged
Plasmid:
限制性酶切位點:
Tag序列:
序列資訊:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
小鼠 CKB / Creatine kinase B type 基因全長cDNA ORF克隆 (表達載體) on other vectors
小鼠 CKB / Creatine kinase B type 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤MG51386-ACGNT$8150
小鼠 CKB / Creatine kinase B type 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤MG51386-ACRNT$8150
小鼠 CKB / Creatine kinase B type 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤MG51386-ANGNT$8150
小鼠 CKB / Creatine kinase B type 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤MG51386-ANRNT$8150
小鼠 CKB / Creatine kinase B type 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤MG51386-CFNT$7060
小鼠 CKB / Creatine kinase B type 基因全長cDNA ORF克隆 (表達載體), C-His 標籤MG51386-CHNT$7060
小鼠 CKB / Creatine kinase B type 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤MG51386-CMNT$7060
小鼠 CKB / Creatine kinase B type 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤MG51386-CYNT$7060
小鼠 CKB / Creatine kinase B type 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤MG51386-NFNT$7060
小鼠 CKB / Creatine kinase B type 基因全長cDNA ORF克隆 (表達載體), N-His 標籤MG51386-NHNT$7060
小鼠 CKB / Creatine kinase B type 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤MG51386-NMNT$7060
小鼠 CKB / Creatine kinase B type 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤MG51386-NYNT$7060
小鼠 CKB / Creatine kinase B type 基因全長cDNA ORF(克隆載體)MG51386-UNT$2720
小鼠 CKB / Creatine kinase B type 基因全長cDNA ORF克隆 (表達載體)MG51386-UTNT$7060
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研究背景

CKB(Creatine kinase B type) contains 1 phosphagen kinase C-terminal domain and 1 phosphagen kinase N-terminal domain. It belongs to the ATP:guanido phosphotransferase family. CKB consists of a homodimer of two identical brain-type CK-B subunits. CKB is a cytoplasmic enzyme involved in cellular energy homeostasis, with certain fractions of the enzyme being bound to cell membranes, ATPases, and a variety of ATP-requiring enzymes in the cell. There, CKB forms tightly coupled microcompartments for in situ regeneration of ATP that has been used up. CKB reversibly catalyzes the transfer of "energy-rich" phosphate between ATP and creatine or between phospho-creatine (PCr) and ADP. Its functional entity is a homodimer in brain, smooth muscle as well as in other tissues and cells such as neuronal cells, retina, kidney, bone etc.

參考資料
  • Wienker TF, et al. (1985) A dominant mutation causing ectopic expression of the creatine kinase B gene maps on chromosome 14. Cytogenet Cell Genet. 40:776.
  • Mariman EC, et al. (1989) Complete nucleotide sequence of the human creatine kinase B gene. Nucleic Acids Res. 17(15):6385.
  • Bong S, et al. (2008) Structural studies of human brain-type creatine kinase complexed with the ADP–Mg2+–NO3−–creatine transition-state analogue complex. FEBS Letters. 582(28): 3959-65.
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    貨號: MG51386-UT
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