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小鼠 LASS2/ceramide synthase 2 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤

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Mouse CERS2 cDNA Clone產品資訊
註冊碼:NM_029789.1
cDNA基因長度:1143bp
cDNA基因描述:Full length Clone DNA of Mus musculus ceramide synthase 2 with C terminal Myc tag.
基因別名:TRH3; Lass2; AI225939; 0610013I17Rik
分子種屬:Mouse
載體:pCMV3-C-Myc
Plasmid:
限制性酶切位點:
Tag序列:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
序列資訊:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

小鼠 LASS2/ceramide synthase 2 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤 on other vectors
小鼠 LASS2/ceramide synthase 2 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤MG51624-ACGNT$8150
小鼠 LASS2/ceramide synthase 2 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤MG51624-ACRNT$8150
小鼠 LASS2/ceramide synthase 2 基因全長cDNA ORF克隆 (表達載體), N-GFPSpark 標籤MG51624-ANGNT$8150
小鼠 LASS2/ceramide synthase 2 基因全長cDNA ORF克隆 (表達載體), N-OFPSpark 標籤MG51624-ANRNT$8150
小鼠 LASS2/ceramide synthase 2 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤MG51624-CFNT$7060
小鼠 LASS2/ceramide synthase 2 基因全長cDNA ORF克隆 (表達載體), C-His 標籤MG51624-CHNT$7060
小鼠 LASS2/ceramide synthase 2 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤MG51624-CMNT$7060
小鼠 LASS2/ceramide synthase 2 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤MG51624-CYNT$7060
Mouse CERS2 Gene cDNA clone plasmidMG51624-GNT$6920
小鼠 LASS2/ceramide synthase 2 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤MG51624-NFNT$7060
小鼠 LASS2/ceramide synthase 2 基因全長cDNA ORF克隆 (表達載體), N-His 標籤MG51624-NHNT$7060
小鼠 LASS2/ceramide synthase 2 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤MG51624-NMNT$7060
小鼠 LASS2/ceramide synthase 2 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤MG51624-NYNT$7060
小鼠 LASS2/ceramide synthase 2 基因全長cDNA ORF(克隆載體)MG51624-UNT$2720
小鼠 LASS2/ceramide synthase 2 基因全長cDNA ORF克隆 (表達載體)MG51624-UTNT$7060
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