CD5L ELISA Kit, Mouse

Cat: KIT50020
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CD5L ELISA Kit, Mouse General Information
Product name
CD5L ELISA Kit, Mouse
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
6.82 pg/mL
Assay range
15.63-1000 pg/mL
Specificity
Recognizes both recombinant and natural Mouse CD5L
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 206.00 415.00 818.00
SD 5.54 21.34 46.07
CV(%) 2.7% 5.1% 5.6%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 208.00 405.00 794.00
SD 4.18 10.33 18.50
CV(%) 2.0% 2.6% 2.3%
Recovery
The recovery of Mouse CD5L spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample Average % Recovery Range
serum (n=3) 103 94 -109%
Linearity
Serum
  Recovery of detected
1:2 91%
1:4 100%
1:8 113%
1:16 86%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This CD5L ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD5L protein in Serum . It contains recombinant Mouse CD5L, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
CD5L ELISA Kit, Mouse Images
Mouse CD5L ELISA Kit Standard Curve 122
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Mouse CD5L ELISA Kit Specificity 185
This assay recognizes both recombinant and natural Mouse CD5L. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
CD5L ELISA Kit, Mouse Alternative Names
42010 ELISA Kit, Mouse;AAC-11 ELISA Kit, Mouse;AI047839 ELISA Kit, Mouse;Api6 ELISA Kit, Mouse;CT2 ELISA Kit, Mouse;Pdp ELISA Kit, Mouse;Sp-alpha ELISA Kit, Mouse
CD5L Background Information

CD5L, also known as CD5 antigen-like, is a soluble protein belonging to group B of the scavenger receptor cysteine-rich (SRCR) superfamily and contains three SRCR domains. It is a secreted glycoprotein and expressed by macrophages presentin lymphoid tissues (spleen, lymph node, thymus, and bone marrow). It binds to myelomonocytic and lymphoid cells and may play an important role in the regulation of the innate and adaptive immune systems. CD5L functions as a pattern recognition molecule by binding both lipoteichoic acid (LTA) on Gram positive and lipopolysaccharide (LPS) on Gram negative bacteria. and the SRCR domain 1 of CD5L retains both the LPS and LTA binding activities. In addtion, it is revealed that CD5L seems to play a role as an inhibitor of apoptosis.

Full Name
CD5 molecule-like
References
  • Resnick, D. et al., 1994, Trends Biochem. Sci. 19: 5-8.
  • Gebe, J. A. et al., 1997, J. Biol. Chem. 272 (10): 6151–6158.
  • Sarrias, M.R. et al., 2004, Crit. Rev. Immunol. 24: 1-37.
  • Mukhopadhyay, S. and Gordon, S., 2004, Immunobiology 209: 39-49.
  • Sarrias, M. R. et al.,2005, J. Biol. Chem. 280 (42): 35391–35398.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
    CD5L Matched ELISA Antibody Pair Set,Mouse 6.25 pg/ml SEK50020
    info info
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