CD48 ELISA Kit, Mouse

Cat: KIT50415
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CD48 ELISA Kit, Mouse General Information
Product name
CD48 ELISA Kit, Mouse
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Lysate
Sensitivity
16.01 pg/mL
Assay range
62.5-4000 pg/mL
Specificity
Recognizes both recombinant and natural Mouse CD48
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 643.00 1,320.00 2,617.00
SD 25.43 52.44 97.33
CV(%) 4.0% 4.0% 3.7%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 729.00 1,507.00 2,956.00
SD 67.53 113.31 193.14
CV(%) 9.3% 7.5% 6.5%
Recovery
The recovery of Mouse CD48 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample Average % Recovery Range
serum (n=3) 84 81 -87%
supernatant (n=3) 100 91 -105%
urine (n=3) 84 81 -87%
Linearity
Supernatant
  Recovery of detected
1:1 93%
1:2 98%
1:4 109%
1:8 95%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This CD48 ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD48 protein in Lysate . It contains recombinant Mouse CD48, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
CD48 ELISA Kit, Mouse Images
Mouse CD48/Blast-1 ELISA Kit Standard Curve 133
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Mouse CD48/Blast-1 ELISA Kit Specificity 186
This assay recognizes both recombinant and natural Mouse CD48. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
CD48 ELISA Kit, Mouse Alternative Names
AI449234 ELISA Kit, Mouse;AW610730 ELISA Kit, Mouse;Bcm-1 ELISA Kit, Mouse;BCM1 ELISA Kit, Mouse;BLAST ELISA Kit, Mouse;BLAST-1 ELISA Kit, Mouse;BLAST1 ELISA Kit, Mouse;MEM-102 ELISA Kit, Mouse;Sgp-60 ELISA Kit, Mouse;SLAMF2 ELISA Kit, Mouse
CD48 Background Information

Cluster of Differentiation 48 (CD48), also known as SLAMF2, BCM-1 and BLAST-1, is a GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 protein performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses.

Immune Checkpoint
Immune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: IHC Antibodies   Immune Checkpoint Detection: ICC Antibodies   Immune Checkpoint Detection: IP Antibodies   Immune Checkpoint Detection: FCM Antibodies   Immune Checkpoint Detection: WB Antibodies
Immune Checkpoint Proteins
Immune Checkpoint Targets   Co-inhibitory Immune Checkpoint Targets

Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
CD48 molecule
References
  • Messmer B, et al. (2006) CD48 stimulation by 2B4 (CD244)-expressing targets activates human NK cells. J Immunol. 176(8): 4646-50
  • Milstein O, et al. (2008) Nanoscale increases in CD2-CD48-mediated intermembrane spacing decrease adhesion and reorganize the immunological synapse. J Biol Chem. 283(49): 34414-22.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
    CD48 Matched ELISA Antibody Pair Set,Mouse 46.875 pg/ml SEK50415
    CD48 ELISA Kit, Human Serum KIT10797
    CD48 Matched ELISA Antibody Pair Set,Human 9.38 pg/ml SEKA10797
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