( We provide with CD19 qPCR primers for gene expression analysis, MP200503 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|小鼠 CD19/B4/CVID3 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||MG50510-ACG|
|小鼠 CD19/B4/CVID3 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||MG50510-ACR|
|小鼠 CD19/B4/CVID3 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||MG50510-CF|
|小鼠 CD19/B4/CVID3 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||MG50510-CH|
|小鼠 CD19/B4/CVID3 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||MG50510-CM|
|小鼠 CD19/B4/CVID3 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||MG50510-CY|
|小鼠 CD19/B4/CVID3 基因全長cDNA ORF(克隆載體)||MG50510-M|
|小鼠 CD19/B4/CVID3 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||MG50510-NF|
|小鼠 CD19/B4/CVID3 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||MG50510-NH|
|小鼠 CD19/B4/CVID3 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||MG50510-NM|
|小鼠 CD19/B4/CVID3 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||MG50510-NY|
|小鼠 CD19/B4/CVID3 基因全長cDNA ORF克隆 (表達載體)||MG50510-UT|
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 19 (CD19) is a member of CD system. CD19 is a cell surface molecule that assembles with the antigen receptor of B-cells. This results in a descent in threshold for antigen receptor-dependent stimulation. A simplified view holds that the ability of B-cells to respond to the various antigens in a specific and sensitive manner is achieved in the presence of low-affinity antigen receptors. CD19 primarily acts as a B-cell coreceptor in conjunction with CD21 and CD81. The formation of the receptor complex is induced by antigen and CD19, induced by exogenous antigen, has been found cytoplasmic tail phosphorylated and bind to sIg.