|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 CD157/BST1 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||MG50319-ACG|
|小鼠 CD157/BST1 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||MG50319-ACR|
|小鼠 CD157/BST1 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||MG50319-CF|
|小鼠 CD157/BST1 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||MG50319-CH|
|小鼠 CD157/BST1 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||MG50319-CM|
|小鼠 CD157/BST1 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||MG50319-CY|
|小鼠 CD157/BST1 基因全長cDNA ORF(克隆載體)||MG50319-M|
|小鼠 CD157/BST1 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||MG50319-NF|
|小鼠 CD157/BST1 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||MG50319-NH|
|小鼠 CD157/BST1 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||MG50319-NM|
|小鼠 CD157/BST1 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||MG50319-NY|
|小鼠 CD157/BST1 基因全長cDNA ORF克隆 (表達載體)||MG50319-UT|
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD157, also known as ADP-ribosyl cyclase 2, is an ectoenzyme sharing several characteristics with ADP-ribosyl cyclase CD38. CD157 was originally identified as a bone marrow stromal cell molecule (BST-1) with a glycosylphosphatidylinositol (GPI) anchor to bind to the cell surface. CD157 is prevalently expressed by cells of the myeloid lineage. CD157 could act as a receptor with signal transduction capability. Further, it regulates calcium homeostasis and promotes polarization in neutrophils and mediates superoxide (O2−) production in the human U937 myeloid line.