CD132 ELISA Kit, Mouse

Cat: KIT50087
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CD132 ELISA Kit, Mouse General Information
Product name
CD132 ELISA Kit, Mouse
Assay type
Solid Phase Sandwich ELISA (quantitative)
Conjugate
HRP
Sample type
Serum
Sensitivity
15.53 pg/mL
Assay range
31.25-2000 pg/mL
Specificity
Recognizes both recombinant and natural Mouse CD132
Interference
Preparations of the factors listed below at 20 ng/mL in a mid-range recombinant mouse IL2RG control were assayed for interference. No significant interference was observed. Interference was observed with recombinant mouse IL2 ,mouse IL13RA1 and mouse IL13RA2 at concentrations ≥ 2 ng/mL.
Precision
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.21 0.40 0.75
SD 0.01 0.01 0.02
CV(%) 3.0% 3.6% 2.0%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.20 0.38 0.75
SD 0.02 0.03 0.06
CV(%) 11.1% 7.9% 8.0%
Recovery
The recovery of Mouse IL2RG spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample Average % Recovery Range
serum (n=3) 120.843828715365 115-126%
Linearity
Serum
  Recovery of detected
1:2 82%
1:4 97%
1:8 116%
1:16 105%
Materials provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Product overview
This CD132 ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD132 protein in Serum . It contains recombinant Mouse CD132, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Shipping
This ELISA Kit is shipped at ambient temperature.
Storage
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
CD132 ELISA Kit, Mouse Images
Mouse CD132 / IL2RG ELISA Kit Standard Curve 124
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Mouse CD132 / IL2RG ELISA Kit Specificity 177
This assay recognizes both recombinant and natural Mouse IL2RG. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
CD132 ELISA Kit, Mouse Alternative Names
CD132 ELISA Kit, Mouse;gamma(c) ELISA Kit, Mouse;gc ELISA Kit, Mouse;[g]c ELISA Kit, Mouse
CD132 Background Information

The common gamma chain (γc) (or CD132), also known as interleukin-2 receptor subunit gamma or IL2RG, is a member of the type I cytokine receptor family expressed on most lymphocyte (white blood cell) populations, and its gene is found on the X-chromosome of mammals. The common gamma chain (γc) (or IL2RG), is a cytokine receptor sub-unit that is common to the receptor complexes for at least six different interleukin receptors: IL-2, IL-4, IL-7, IL-9, IL-15 and interleukin-21 receptor. It is a component of multiple cytokine receptors that are essential for lymphocyte development and function. X-linked severe combined immunodeficiency (XSCID) is a rare and potentially fatal disease caused by mutations of IL2RG, the gene encoding IL2RG. IL2RG was demonstrated to be a component of the IL-4 receptor on the basis of chemical cross-linking data, the ability of IL2RG to augment IL-4 binding affinity. The observation that IL-2R gamma is a functional component of the IL-4 receptor, together with the finding that IL-2R gamma associates with the IL-7 receptor, begins to elucidate why deficiency of this common gamma chain (gamma c) has a profound effect on lymphoid function and development, as seen in X-linked severe combined immunodeficiency.

Full Name
interleukin 2 receptor, gamma
References
  • Russell SM, et al. (1993) Interleukin-2 receptor gamma chain: a functional component of the interleukin-4 receptor. Science. 262 (5141): 1880-3.
  • Miyazaki T, et al. (1994) Functional activation of Jak1 and Jak3 by selective association with IL-2 receptor subunits. Science. 266 (5187): 1045-7.
  • Takeshita T, et al. (1992) Cloning of the gamma chain of the human IL-2 receptor. Science. 257 (5068): 379-82.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
    CD132 Matched ELISA Antibody Pair Set,Mouse 24 pg/ml SEK50087
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