|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 CD10/Neprilysin 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||MG50218-ACG|
|小鼠 CD10/Neprilysin 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||MG50218-ACR|
|小鼠 CD10/Neprilysin 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||MG50218-CF|
|小鼠 CD10/Neprilysin 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||MG50218-CH|
|小鼠 CD10/Neprilysin 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||MG50218-CM|
|小鼠 CD10/Neprilysin 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||MG50218-CY|
|小鼠 CD10/Neprilysin 基因全長cDNA ORF(克隆載體)||MG50218-G|
|小鼠 CD10/Neprilysin 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||MG50218-NF|
|小鼠 CD10/Neprilysin 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||MG50218-NH|
|小鼠 CD10/Neprilysin 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||MG50218-NM|
|小鼠 CD10/Neprilysin 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||MG50218-NY|
|小鼠 CD10/Neprilysin 基因全長cDNA ORF克隆 (表達載體)||MG50218-UT|
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 10 (CD10), also known as Neprilysin and neutral endopeptidase, is a member of the CD system. CD10 is a zinc-dependent metalloprotease enzyme that had function to degrade a number of small secreted peptides such as the amyloid beta peptide. It exist as a membrane-bound protein and have high concentration in kidney and lung tissues. Mutations in the CD10 gene can induce the familial forms of Alzheimer's disease, providing strong evidence for the protein's association with the Alzheimer's disease process. CD10 is also associated with other biochemical processes.