|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 BLK Kinase 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||MG50365-ACG|
|小鼠 BLK Kinase 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||MG50365-ACR|
|小鼠 BLK Kinase 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||MG50365-CF|
|小鼠 BLK Kinase 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||MG50365-CH|
|小鼠 BLK Kinase 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||MG50365-CM|
|小鼠 BLK Kinase 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||MG50365-CY|
|小鼠 BLK Kinase 基因全長cDNA ORF(克隆載體)||MG50365-M|
|小鼠 BLK Kinase 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||MG50365-NF|
|小鼠 BLK Kinase 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||MG50365-NH|
|小鼠 BLK Kinase 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||MG50365-NM|
|小鼠 BLK Kinase 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||MG50365-NY|
|小鼠 BLK Kinase 基因全長cDNA ORF克隆 (表達載體)||MG50365-UT|
Tyrosine-protein kinase Blk, also known as B lymphocyte kinase, p55-Blk and BLK, is a member of the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. BLK / p55-Blk is expressed in lymphatic organs, pancreatic islets, Leydig cells, striate ducts of salivary glands and hair follicles. BLK / p55-Blk is a src-family protein tyrosine kinase specifically expressed in B-lineage cells of mice. The early onset of Blk expression during B-cell development in the bone marrow and the high expression levels of Blk in mature B cells suggest a possible important role of Blk in B-cell physiology. It is a modulator of beta-cells function, acting through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose. Defects in BLK are a cause of maturity-onset diabetes of the young type 11 which is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.