|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|小鼠 BCHE/Butyrylcholinesterase 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||MG50418-ACG|
|小鼠 BCHE/Butyrylcholinesterase 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||MG50418-ACR|
|小鼠 BCHE/Butyrylcholinesterase 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||MG50418-CF|
|小鼠 BCHE/Butyrylcholinesterase 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||MG50418-CH|
|小鼠 BCHE/Butyrylcholinesterase 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||MG50418-CM|
|小鼠 BCHE/Butyrylcholinesterase 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||MG50418-CY|
|小鼠 BCHE/Butyrylcholinesterase 基因全長cDNA ORF(克隆載體)||MG50418-M|
|小鼠 BCHE/Butyrylcholinesterase 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||MG50418-NF|
|小鼠 BCHE/Butyrylcholinesterase 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||MG50418-NH|
|小鼠 BCHE/Butyrylcholinesterase 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||MG50418-NM|
|小鼠 BCHE/Butyrylcholinesterase 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||MG50418-NY|
|小鼠 BCHE/Butyrylcholinesterase 基因全長cDNA ORF克隆 (表達載體)||MG50418-UT|
Butyrylcholinesterase (BCHE), also known as cholinesterase or BuChE, is an enzyme defined as "pseudo" or "non-neuronal" cholinesterase. Butyrylcholinesterase (BCHE) is widely distributed in the nervous system as well as blood plasma. It is constitutively similar to the neuronal acetylcholinesterase, and is a non-specific cholinesterase which hydrolyses many different choline esters. Butyrylcholinesterase (BCHE) is a glycoprotein of 4 identical subunits, that were arranged as a dimer of dimers with each dimer composed of two identical subunits joined by interchain disulfide bonds. Butyrylcholinesterase (BCHE) behaves principally similar to the true enzyme and thus can play a similar role in nerve conduction, although it participates probably only in relatively slow conductive processes and could be involved in other nervous system functions and in neurodegenerative diseases. It can hydrolyze toxic esters such as cocaine or scavenge organophosphorus pesticides and nerve agents. Purified human serum cholinesterase combines in its active surface an anionic and an esteratic site, similar to true cholinesterase. It has been demonstrated that butyrylcholinesterase (BCHE) may have a greater role in cholinergic transmission than previously surmised, making BChE inhibition an important therapeutic goal in Alzheimer's disease.