( We provide with TNFSF13B qPCR primers for gene expression analysis, MP200373 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|小鼠 BAFF/BLyS/TNFSF13B 基因全長cDNA ORF克隆 (表達載體), C-GFPSpark 標籤||MG50352-ACG|
|小鼠 BAFF/BLyS/TNFSF13B 基因全長cDNA ORF克隆 (表達載體), C-OFPSpark 標籤||MG50352-ACR|
|小鼠 BAFF/BLyS/TNFSF13B 基因全長cDNA ORF克隆 (表達載體), C-Flag 標籤||MG50352-CF|
|小鼠 BAFF/BLyS/TNFSF13B 基因全長cDNA ORF克隆 (表達載體), C-His 標籤||MG50352-CH|
|小鼠 BAFF/BLyS/TNFSF13B 基因全長cDNA ORF克隆 (表達載體), C-Myc 標籤||MG50352-CM|
|小鼠 BAFF/BLyS/TNFSF13B 基因全長cDNA ORF克隆 (表達載體), C-HA 標籤||MG50352-CY|
|小鼠 BAFF/BLyS/TNFSF13B 基因全長cDNA ORF(克隆載體)||MG50352-M|
|小鼠 BAFF/BLyS/TNFSF13B 基因全長cDNA ORF克隆 (表達載體), N-Flag 標籤||MG50352-NF|
|小鼠 BAFF/BLyS/TNFSF13B 基因全長cDNA ORF克隆 (表達載體), N-His 標籤||MG50352-NH|
|小鼠 BAFF/BLyS/TNFSF13B 基因全長cDNA ORF克隆 (表達載體), N-Myc 標籤||MG50352-NM|
|小鼠 BAFF/BLyS/TNFSF13B 基因全長cDNA ORF克隆 (表達載體), N-HA 標籤||MG50352-NY|
|小鼠 BAFF/BLyS/TNFSF13B 基因全長cDNA ORF克隆 (表達載體)||MG50352-UT|
B lymphocyte stimulator (BLyS), also known as TNFSF13B, CD257 and BAFF, is single-pass type II membrane protein, which belongs to the tumor necrosis factor family. BAFF is abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. BAFF is a cytokine and serves as a ligand for receptors TNFRSF13B (TACI), TNFRSF17 (BCMA), and TNFRSF13C (BAFFR). These receptors is a prominent factor in B cell differentiation, homeostasis, and selection. BLyS levels affect survival signals and selective apoptosis of autoantibody-producing B cells. Thus, it acts as a potent B cell activator and has been shown to play an important role in the proliferation and differentiation of B cells. Overexpression of BLyS in mice can lead to clinical and serological features of systemic lupus erythematosus (SLE) and Sjögren's syndrome (SS). BLyS as an attractive therapeutic target in human rheumatic diseases. The ability of BLyS to regulate both the size and repertoire of the peripheral B cell compartment raises the possibility that BLyS and antagonists thereof may form the basis of a therapeutic trichotomy. As an agonist, BLyS protein may enhance humoral immunity in congenital or acquired immunodeficiencies such as those resulting from viral infection or cancer therapy.