MICB Protein, Human, Recombinant (His Tag)

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MICB Protein, Human, Recombinant (His Tag): Product Information

Purity
> 98 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
Immobilized human MICB-His (Cat:10759-H08H) at 10 μg/ml (100 μl/well) can bind S4-Fc3L3-NKG2D (Cat:10575-H01S), The EC50 of S4-Fc3L3-NKG2D (Cat:10575-H01S) is 0.52-1.2 μg/ml.
Protein Construction
A DNA sequence encoding the extracellular domain of human MICB (NP_005922.2) (Met 1-Gly 298) was expressed with a C-terminal polyhistidine tag.
Accession#
Expressed Host
HEK293 Cells
Species
Human
Predicted N Terminal
Ala 23
Molecule Mass
The recombinant human MICB consists of 287 amino acids and has a predicted molecular mass of 33 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh MICB is approximately 45-50 kDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

MICB Protein, Human, Recombinant (His Tag): Images

Immobilized human MICB-His (Cat:10759-H08H) at 10 μg/ml (100 μl/well) can bind S4-Fc3L3-NKG2D (Cat:10575-H01S), The EC50 of S4-Fc3L3-NKG2D (Cat:10575-H01S) is 0.52-1.2 μg/ml.

MICB Protein, Human, Recombinant (His Tag): Alternative Names

PERB11.2 Protein, Human

MICB Background Information

MHC class I polypeptide-related sequence B, also known as MICB, is a heavily glycosylated protein serving as a ligand for the type II receptor NKG2D. MICB shares 85% amino acid identity with MICA, a closely related protein, both of which contain three extracellular immunoglobulin-like domains, but without capacity to bind peptide or interact with beta-2-microglobulin. acting as a stress-induced self-antigen, binding of MICB to the NKG2D receptor activates the cytolytic response of natural killer (NK) cells, CD8+αβ T cells, and γδ T cells on which the receptor is expressed. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B recognition thus is involved in tumor surveillance, viral infections, and autoimmune diseases.
Full Name
MHC class I polypeptide-related sequence B
References
  • Bauer, S. et al., 1999, Science. 285:727-729.
  • Braud, V.M. et al., 1999, Curr. Opin. Immunol. 11: 100-108.
  • Groh, V. et al., 2001, Nature Immunol. 2: 255-260.
  • Stephens, H., 2001, Trends Immunol. 22: 378-385.
  • Borrego, F. et al., 2002, Mol. Immunol. 38: 637–660.
  • Groh, V. et al., 2002, Nature. 419:734-738.
  • Mic-binding antibodies and methods of use thereof
    Author
    JD Wu
    Year
    2012
    Journal
    US 20120315287 A1
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