MD1 cDNA ORF Clone, Human, C-DDK (Flag®) tag

Cat: HG10242-M-F
Contact us:
215-583-7898
order@sinobiological.com
Distributors
All MD1 Reagents
CRO Services

All MD1 Reagents
MD1 cDNA ORF Clone, Human, C-DDK (Flag®) tag General Information
Gene
Species
Human
NCBI Ref Seq
RefSeq ORF Size
489 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence.
Description
Full length Clone DNA of Human lymphocyte antigen 86 with Flag tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV2-FLAG
Restriction Sites
KpnI + XhoI (5.4kb + 0.54kb)
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
MD1 cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

MD1 cDNA ORF Clone, Human, C-DDK (Flag®) tag Alternative Names
dJ80N2.1 cDNA ORF Clone, Human;MD-1 cDNA ORF Clone, Human;MD1 cDNA ORF Clone, Human;MMD-1 cDNA ORF Clone, Human
MD1 Background Information

MD-1 and MD-2 are secretory glycoproteins that exist on the cell surface in complexes with transmembrane proteins. MD-1 is anchored by radioprotective 105 (RP105) which is a molecule containing leucine-rich repeats and is expressed on B cells, dentritic cells and macrophages, while MD-2 is associated with TLR4. MD-1 is required for efficient RP105 cell surface expression and function. It is indicated that the RP105/MD1 complex, in conjunction with TLR4, mediates the innate immune response to LPS in B cells, and also plays a role in protecting against apoptosis, B-cell proliferation, etc. Mouse MD-1 cDNA encodes a 162 amino acid precursor protein with a putative 19 aa signal peptide and two potential N-linked glycosylation sites. It shares 40% and 66% amino acid sequence identity with chicken and human MD-1 respectively. MD-1 is mainly expressed in spleen, and also detectable in liver, brain, thymus, and kidney.

Full Name
lymphocyte antigen 86
References
  • Miura Y., et al.,(1998), RP105 is associated with MD-1 and transmits an activation signal in human B cells. Blood 92:2815-2822.
  • Begum N.A., et al., (1999), Human MD-1 homologue is a BCG-regulated gene product in monocytes: Its identification by differential display.Biochem. Biophys. Res. Commun. 256:325-329.
  • Mungall A.J., et al.,(2003), The DNA sequence and analysis of human chromosome 6.Nature 425:805-811.
  • MD1 cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    MD1 cDNA ORF Clone in Cloning Vector, Mouse MG50062-M
    MD1 cDNA ORF Clone, Mouse, untagged MG50062-UT
    MD1 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50062-ACG
    MD1 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50062-ACGLN
    MD1 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50062-ACR
    MD1 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50062-CF
    MD1 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50062-NF
    MD1 cDNA ORF Clone, Mouse, C-His tag MG50062-CH
    MD1 cDNA ORF Clone, Mouse, N-His tag MG50062-NH
    MD1 cDNA ORF Clone, Mouse, C-HA tag MG50062-CY
    MD1 cDNA ORF Clone, Mouse, N-HA tag MG50062-NY
    MD1 cDNA ORF Clone, Mouse, C-Myc tag MG50062-CM
    MD1 cDNA ORF Clone, Mouse, N-Myc tag MG50062-NM
    MD1 qPCR Primer Pairs, Mouse MP200062

    MD1 cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    MD1 cDNA ORF Clone in Cloning Vector, Human HG10242-M
    MD1 cDNA ORF Clone, Human, untagged HG10242-UT
    MD1 cDNA ORF Clone, Human, C-GFPSpark® tag HG10242-ACG
    MD1 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10242-ACGLN
    MD1 cDNA ORF Clone, Human, C-OFPSpark® tag HG10242-ACR
    MD1 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10242-CF
    MD1 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10242-NF
    MD1 cDNA ORF Clone, Human, C-His tag HG10242-CH
    MD1 cDNA ORF Clone, Human, N-His tag HG10242-NH
    MD1 cDNA ORF Clone, Human, C-HA tag HG10242-CY
    MD1 cDNA ORF Clone, Human, N-HA tag HG10242-NY
    MD1 cDNA ORF Clone, Human, C-Myc tag HG10242-CM
    MD1 cDNA ORF Clone, Human, N-Myc tag HG10242-NM
    MD1 qPCR Primer Pairs, Human HP100292
    info info
    添加购物车成功! 添加购物车失败!