Anti-LAMP1 Antibody

Cat: 11215-R107

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Anti-LAMP1 Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-LAMP1 Antibody
Validated applications
WB,ELISA,IHC-P,FCM,ICC/IF,IF,IP
Species reactivity
Reacts with: Human
Specificity
Human LAMP1
Immunogen
Recombinant Human LAMP1 protein (Catalog#11215-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human LAMP1 extracellular domain (rh LAMP1; Catalog#11215-H08H; NP_005552.3; Met 1-Met 382).
Source
Monoclonal Rabbit IgG Clone #107
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Anti-LAMP1 Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
IHC-P 1:500-1:2500  
ICC-IF 1:100-1:500  
FCM 1:100-1:500  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-LAMP1 Antibody (Rabbit Monoclonal antibody) Images

Human LAMP-1/CD107a/LAMP1 Immunohistochemistry(IHC) 3952
Immunochemical staining of human LAMP1 in human placenta with rabbit monoclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
Human LAMP-1/CD107a/LAMP1 Immunohistochemistry(IHC) 3954
Immunochemical staining of human LAMP1 in human breast carcinoma with rabbit monoclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Human LAMP-1/CD107a/LAMP1 Flow Cytometry (FC) 5109
Flow cytometric analysis of Human LAMP1(CD107a) on Jurkat cells. Cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human LAMP1(CD107a), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Human LAMP-1/CD107a/LAMP1 Immunohistochemistry(IHC) 5476
Confocal immunofluorescence analysis of Human LAMP1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human LAMP1 monoclonal antibody (1:300). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody, countstained with Alexa Fluor® 546-conjugated phallotoxins (red) and DAPI (blue). Positive staining was localized to lysosome membrane.
Human LAMP-1/CD107a/LAMP1 Western blot (WB) 13367

Anti-LAMP1 rabbit monoclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:120 kDa

Observed band size:120 kDa

Human LAMP-1/CD107a/LAMP1 Immunoprecipitation(IP) 17685

LAMP1 was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

1 µL anti-LAMP1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-LAMP1 rabbit monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 45 kDa

Observed band size: 113 kDa

Anti-LAMP1 Antibody Alternative Names

Anti-CD107a Antibody;Anti-LAMPA Antibody;Anti-LGP120 Antibody

LAMP1 Background Information

Lysosome-associated membrane glycoprotein 1, also known as CD17 antigen-like family member A, CD17a, and LAMP1, is a single-pass type I membrane protein which belongs to the LAMP family. CD17a is expressed largely in the endosome-lysosome membranes of cells, but is also found on the plasma membrane (1-2% of total LAMP1). LAMP1 has been implicated in a variety of cellular functions, including cancer metastasis. It has been proposed LAMP1 serves as a therapeutic agent for some cancers, as well as a marker for lysosomal storage disorders and different cell types such as cytotoxic T cells. LAMP2, also known as CD17b, may also play a role in tumor cell metastasis and functions in the protection, maintenance, and adhesion of the lysosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation. LAMP-1 is a glycoprotein highly expressed in lysosomal membranes. The present study was initiated to test LAMP-1 mRNA and protein levels in post mortem frontal cortex (area 8) of Alzheimer's disease (AD) stages I-IIA/B and stages V-VIC of Braak and Braak, compared with age-matched controls. LAMP-1 occurred in microglia and multinucleated giant cells in one AD case in whom amyloid burden was cleared following betaA-peptide immunization. In addition, LAMP-1 has been suggested to be a cell surface receptor for a specific amelogenin isoform, leucine-rich amelogenin peptide or LRAP. LAMP-1 can serve as a cell surface binding site for amelogenin on dental follicle cells and cementoblasts.
Full Name
lysosomal-associated membrane protein 1
References
  • Parkinson-Lawrence EJ, et al. (2005) Immunochemical analysis of CD107a (LAMP-1). Cell Immunol. 236(1-2): 161-6.
  • Barrachina M, et al. (2006) Lysosome-associated membrane protein 1 (LAMP-1) in Alzheimer's disease. Neuropathol Appl Neurobiol. 32(5): 505-16.
  • Zhang H, et al. (2010) Full length amelogenin binds to cell surface LAMP-1 on tooth root/periodontium associated cells. Arch Oral Biol. 55(6): 417-25.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-LAMP1 Antibody Mouse Monoclonal ELISA(Cap) 11215-MM09
    Anti-LAMP1 Antibody (APC) Rabbit Monoclonal FCM 11215-R107-A
    Anti-LAMP1 Antibody Rabbit Polyclonal WB,ELISA,IP 11215-RP02
    Anti-LAMP1 Antibody Mouse Monoclonal WB,ELISA,ELISA(Det),IHC-P,FCM,ICC/IF,IF,IP 11215-MM07
    Anti-LAMP1 Antibody (FITC) Mouse Monoclonal FCM 11215-MM07-F
    Anti-LAMP1 Antibody (PE) Rabbit Monoclonal FCM 11215-R107-P
    Anti-LAMP1 Antibody (APC) Mouse Monoclonal FCM 11215-MM07-A
    Anti-LAMP1 Antibody (FITC) Rabbit Monoclonal FCM 11215-R107-F
    Anti-LAMP1 Antibody (PE) Mouse Monoclonal FCM 11215-MM07-P
    Anti-LAMP1 Antibody Rabbit Polyclonal WB,ELISA 11215-RP01
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