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大鼠 KIRREL3 / NEPH2 蛋白 (His 標籤)

  • Rat KIRREL3 / NEPH2 Protein (His Tag) SDS-PAGE
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大鼠 KIRREL3/NEPH2 蛋白產品資訊
分子別稱:KIRREL3
蛋白構建:A DNA sequence encoding the rat KIRREL3 (Q09GS6) (Met1-Ala523) was expressed with a polyhistidine tag at the C-terminus.
分子種屬:Rat
表達宿主:Human Cells
#N/A:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
大鼠 KIRREL3/NEPH2 蛋白質控檢測
純度:> 95 % as determined by SDS-PAGE
生物活性:Measured by the ability of the immobilized protein to support the adhesion of MS1 mouse pancreatic islet endothelial cells.
When cells are added to rat KIRREL3 coated plates (15 μg/mL, 100 μL/well), > 20% will adhere specifically after 90 minutes at 37 ℃.
內毒素:< 1.0 EU per μg of the protein as determined by the LAL method
穩定性:Samples are stable for up to twelve months from date of receipt at -70℃
預測N端:Leu 22
分子量:The recombinant rat KIRREL3 comprises 513 amino acids and predicts a molecular mass of 56.4 kDa. The apparent molecular mass of the recombinant protein is approximately 60-64 kDa in SDS-PAGE under reducing conditions due to glycosylation.
緩衝液:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
大鼠 KIRREL3/NEPH2 蛋白使用指導
儲存方法:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
#N/A:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
大鼠 KIRREL3/NEPH2 Protein SDS-PAGE
Rat KIRREL3 / NEPH2 Protein (His Tag) SDS-PAGE

KIRREL3/NEPH2 相關信號通路

    Other KIRREL3/NEPH2 Recombinant Protein Products
    80225-R08H
    KIRREL3/NEPH2 研究背景

    Kin of IRRE-like protein 3 (KIRREL3) also known as nin of irregular chiasm-like protein 3 or nephrin-like protein 2 (NEPH2) is a member of the nephrin-like protein family of transmembrane proteins, which includes NEPH1 (KIRREL) and NEPH3 (KIRREL2). KIRREL3/NEPH2 is expressed in fetalv and adult brain, and also in podocytes of kidney glomeruli. The cytoplasmic domains of KIRREL3/NEPH2 interact with the C-terminus of podocin, also expressed in the podocytes, cells involved in ensuring size- and charge-selective ultrafiltration. Mutations in KIRREL3/NEPH2 are associated with mental retardation autosomal dominant type 4. KIRREL3/NEPH2 expression is turned on in migrating nucleogenesis of the pontine nucleus (PN) neurons only after they enter the presumptive nuclear region. KIRREL3/NEPH2 knockdown disrupted the nuclear organization of PN presumably by changing the migratory behavior of PN neurons inside the nuclear region. Moreover, overexpression of the cytoplasmic region of KIRREL3, which can sequester intracellular signaling of endogenous KIRREL3, resulted in similar phenotypes. Overall, these results suggest KIRREL3 is involved in the nucleogenesis of the PN through the control of neuronal migration inside the nucleus.

    大鼠 KIRREL3/NEPH2 參考資料
  • Bhalla K, et al. (2008) Alterations in CDH15 and KIRREL3 in patients with mild to severe intellectual disability. Am J Hum Genet. 83(6): 703-13.
  • Gerke P, et al. (2005) NEPH2 is located at the glomerular slit diaphragm, interacts with nephrin and is cleaved from podocytes by metalloproteinases. J Am Soc Nephrol. 16(6): 1693-702.
  • Gerke P, et al. (2006) Neuronal expression and interaction with the synaptic protein CASK suggest a role for Neph1 and Neph2 in synaptogenesis. J Comp Neurol. 498(4): 466-75.
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    貨號: 80225-R08H-50
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    Datasheet & Documentation

    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    請注意:所有產品都是“僅用於科研,而不能用於診斷或治療用途”