Anti-JAM-A Antibody

Cat: 10198-MM04

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Anti-JAM-A Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-JAM-A Antibody
Validated applications
FCM,ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human JAM-A
Immunogen
Recombinant Human JAM-A / F11R / CD321 protein (Catalog#10198-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human JAM-A / F11R / CD321 (rh JAM-A / F11R / CD321; Catalog#10198-H08H; NP_058642.1; Met1-Ala242). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #04
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Anti-JAM-A Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution Notes
ICC-IF 1:20-1:100  
FCM 1:25-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-JAM-A Antibody (Mouse Monoclonal antibody) Images

Human Junctional Adhesion Molecule A Immunohistochemistry(IHC) 2898
Immunofluorescence staining of Human JAM-A in A431 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human JAM-A monoclonal antibody (1:100) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated (left panel, captured by laser confocal scanning microscope) and Alexa Fluor®594-conjugated (right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to plasma membrane.
Human Junctional Adhesion Molecule A Flow Cytometry (FC) 5118
Flow cytometric analysis of human JAM-A(CD321) expression on SKBR3 cells. Cells were stained with purified anti-Human JAM-A(CD321), then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.

Anti-JAM-A Antibody Alternative Names

Anti-CD321 Antibody;Anti-JAM Antibody;Anti-JAM1 Antibody;Anti-JAMA Antibody;Anti-JCAM Antibody;Anti-KAT Antibody;Anti-PAM-1 Antibody

JAM-A Background Information

Junctional adhesion molecule-A (JAM-A), also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321), is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells, as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1, involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily, JAM-A protein involves in platelet adhesion, secretion and aggregation, and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition, it may be a potential therapeutic target for breast cancer.
Full Name
F11 receptor
References
  • Guglielmi KM, et al. (2007) Reovirus binding determinants in junctional adhesion molecule-A. J Biol Chem. 282(24): 17930-40.
  • Yeung D, et al. (2008) Decreased junctional adhesion molecule-A expression during blood-brain barrier breakdown. Acta Neuropathol. 115(6): 635-42.
  • Ong KL, et al. (2009) Elevated plasma level of soluble F11 receptor/junctional adhesion molecule-A (F11R/JAM-A) in hypertension. Am J Hypertens. 22(5): 500-5.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-JAM-A Antibody (PE) Mouse Monoclonal FCM 10198-MM04-P
    Anti-JAM-A Antibody Rabbit Polyclonal ELISA 10198-T16
    Anti-JAM-A Antibody (APC) Mouse Monoclonal FCM 10198-MM04-A
    Anti-JAM-A Antibody Rabbit Polyclonal WB,ELISA,IP 10198-RP04
    Anti-JAM-A Antibody (FITC) Mouse Monoclonal FCM 10198-MM04-F
    Anti-JAM-A Antibody Mouse Monoclonal ELISA(Cap) 10198-MM08
    Anti-JAM-A Antibody Rabbit Polyclonal ELISA 10198-RP01
    Anti-JAM-A Antibody Mouse Monoclonal ELISA,ELISA(Det) 10198-MM02
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