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甲型流感 H9N5 (A/shorebird/DE/261/2003) 血凝素 (Hemagglutinin / HA) 蛋白 (His 標籤)

  • Influenza A H9N5 (A/shorebird/DE/261/2003) Hemagglutinin / HA Protein (His Tag)
產品資料評論相關產品實驗方法
 HA 蛋白產品資訊
分子別稱:Hemagglutinin, HA
蛋白構建:A DNA sequence encoding the Influenza A virus (A/shorebird/DE/261/2003(H9N5)) hemagglutinin (ABB87950.1) (Met1-Lys523), termed as HA, was expressed with a polyhistidine tag at the C-terminus.
分子種屬:H9N5
表達宿主:Baculovirus-Insect Cells
#N/A:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
 HA 蛋白質控檢測
純度:> 95 % as determined by SDS-PAGE
生物活性:Measured by its ability to agglutinate guinea pig red blood cells.
HA titer is 0.02-0.1μg/mL for 1%GRBC
內毒素:< 1.0 EU per μg protein as determined by the LAL method.
穩定性:Samples are stable for up to twelve months from date of receipt at -70℃
預測N端:Asp 19
分子量:The recombinant hemagglutinin of Influenza A virus (A/shorebird/DE/261/2003(H9N5)) consists 516 amino acids and predicts a molecular mass of 58.3 kDa.
緩衝液:Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
 HA 蛋白使用指導
儲存方法:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
#N/A:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
 HA Protein SDS-PAGE
Influenza A H9N5 (A/shorebird/DE/261/2003) Hemagglutinin / HA Protein (His Tag)

HA 相關信號通路

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    HA 研究背景

    The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA0, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA0) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

     HA 參考資料
  • White JM, Hoffman LR, Arevalo JH, et al. (1997). "Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.
  • Suzuki Y (March 2005). "Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses". Biol. Pharm. Bull. 28 (3): 399–408.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). "Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential". Avian Dis. 40 (2): 425–37
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    貨號: 40181-V08B-50
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    Datasheet & Documentation

    Contact Us
    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    請注意:所有產品都是“僅用於科研,而不能用於診斷或治療用途”