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甲型流感 H4N4 (mallard duck/Alberta/299/1977) 血凝素 (Hemagglutinin / HA) (Codon Optimized) ORF mammalian expression plasmid, N-His 標籤

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H4N4 HA cDNA Clone產品資訊
註冊碼:
cDNA基因長度:1695bp
cDNA基因描述:Full length Clone DNA of Influenza A H4N4 (mallard duck/Alberta/299/1977) Hemagglutinin with N terminal His tag.
基因別名:Hemagglutinin, HA
分子種屬:H4N4
載體:pCMV3-SP-N-His
Plasmid:
限制性酶切位點:
Tag序列:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
序列資訊:A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with ABB87495.1.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at ambient temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

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