输入关键词搜索之后,可以在左侧选择分子或者产品类型以精简过滤器,快速查找产品。如果有任何问题,请 联系我们!
Text Size:AAA

甲型流感 H1N1 (Beijing/22808/2009) 血凝素 (Hemagglutinin / HA) (Codon Optimized) ORF mammalian expression plasmid, C-HA 標籤

產品資料評論相關產品實驗方法
H1N1 HA cDNA Clone產品資訊
註冊碼:
cDNA基因長度:1701bp
cDNA基因描述:Full length Clone DNA of Influenza A H1N1 (A/Beijing/22808/2009) Hemagglutinin with C terminal HA tag.
基因別名:Hemagglutinin, HA
分子種屬:H1N1
載體:pCMV3-C-HA
Plasmid:
限制性酶切位點:
Tag序列:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
序列資訊:A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with ADD64203.1.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
運輸條件:Each tube contains lyophilized plasmid.
儲存方法:The lyophilized plasmid can be stored at ambient temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

甲型流感 H1N1 (Beijing/22808/2009) 血凝素 (Hemagglutinin / HA) (Codon Optimized) ORF mammalian expression plasmid, C-HA 標籤 on other vectors
甲型流感 H1N1 (A/Beijing/22808/2009) 血凝素 (Hemagglutinin / HA) ORF mammalian expression plasmid (Codon Optimized), C-GFPSpark-taggedVG40035-ACGNT$12500
甲型流感 H1N1 (A/Beijing/22808/2009) 血凝素 (Hemagglutinin / HA) (Codon Optimized) ORF mammalian expression plasmid, C-OFPSpark / RFP 標籤VG40035-ACRNT$12500
甲型流感 H1N1 (A/Beijing/22808/2009) 血凝素 (Hemagglutinin / HA) ORF mammalian expression plasmid (Codon Optimized)VG40035-CNT$11410
甲型流感 H1N1 (A/Beijing/22808/2009) 血凝素 (Hemagglutinin / HA) (Codon Optimized) ORF mammalian expression plasmid, Myc 標籤VG40035-C-MNT$11410
甲型流感 H1N1 (Beijing/22808/2009) 血凝素 (Hemagglutinin / HA) ORF mammalian expression plasmid (Codon Optimized), C-Flag 標籤VG40035-CFNT$11410
甲型流感 H1N1 (Beijing/22808/2009) 血凝素 (Hemagglutinin / HA) (Codon Optimized) ORF mammalian expression plasmid, C-His 標籤VG40035-CHNT$11410
甲型流感 H1N1 (Beijing/22808/2009) 血凝素 (Hemagglutinin / HA) (Codon Optimized) ORF mammalian expression plasmid, C-Myc 標籤VG40035-CMNT$11410
甲型流感 H1N1 (Beijing/22808/2009) 血凝素 (Hemagglutinin / HA) (Codon Optimized) ORF mammalian expression plasmid, C-HA 標籤VG40035-CYNT$11410
甲型流感 H1N1 (Beijing/22808/2009) 血凝素 (Hemagglutinin / HA) (Codon Optimized) ORF mammalian expression plasmid, N-Flag 標籤VG40035-NFNT$11410
甲型流感 H1N1 (Beijing/22808/2009) 血凝素 (Hemagglutinin / HA) (Codon Optimized) ORF mammalian expression plasmid, N-His 標籤VG40035-NHNT$11410
甲型流感 H1N1 (Beijing/22808/2009) 血凝素 (Hemagglutinin / HA) ORF mammalian expression plasmid (Codon Optimized), N-Myc-taggedVG40035-NMNT$11410
甲型流感 H1N1 (Beijing/22808/2009) 血凝素 (Hemagglutinin / HA) (Codon Optimized) ORF mammalian expression plasmid, N-HA 標籤VG40035-NYNT$11410
甲型流感 H1N1 (Beijing/22808/2009) 血凝素 (Hemagglutinin / HA) ORF mammalian expression plasmid (Codon Optimized)VG40035-UTNT$11410
 點擊瞭解更多載體資訊
Product nameProduct name
研究背景

The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA0, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA0) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

參考資料
  • White JM, Hoffman LR, Arevalo JH, et al. (1997). "Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.
  • Suzuki Y (March 2005). "Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses". Biol. Pharm. Bull. 28 (3): 399–408.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). "Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential". Avian Dis. 40 (2): 425–37
  • Size / Price
    貨號: VG40035-CY
    目錄價: 
    單價:      (You Save: )
    規格:
    數量:+-
    庫存2-3 weeks
    大包裝查詢加入購物車
    Contact Us
    產品圖片
        您曾流覽過的產品
          All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
          請注意:所有產品都是“僅用於科研,而不能用於診斷或治療用途”