Anti-UCHL3 Magnetic Beads Immunoprecipitation (IP) Kit

Cat: MB80087-RP02
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Anti-UCHL3 Magnetic Beads-IP Kit Product Components
Components Storage
Anti-UCHL3 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
CD166 Positive Cell Lysate -20℃ for 12 months
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

[1] The IP KIT contains anti-UCHL3 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-UCHL3 Magnetic Beads-IP Kit Product Description
The Anti-UCHL3 magnetic Beads, conjugated with Anti-UCHL3 antibody, are used for immuneprecipitation (IP) of UCHL3 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing UCHL3 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound UCHL3 proteins are dissociated from the magnetic beads using an elution buffer.
Anti-UCHL3 Magnetic Beads-IP Kit Antibody Information
Antibody
Anti-UCHL3 Antibody(80087-RP02)
Immunogen
Recombinant Rat UCHL3 protein (Catalog#80087-R07E)
Species Reactivity
Rat UCHL3
Source
Polyclonal Rat Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Rat UCHL3 (rR UCHL3; Catalog#80087-R07E; Q91Y78; Glu 2-Ala 230). UCHL3 specific IgG was purified by Rat UCHL3 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
UCHL3 Background Information

Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCH-L3, Ubiquitin thioesterase L3 and UCHL3, is a ubiquitin-protein hydrolase which belongs to the peptidase C12 family. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCHL3 is highly expressed in heart, skeletal muscle, and testis. UCHL1 and UCHL3 are two of the deubiquitinating enzymes expressed in the brain. These phenotypes indicate the importance of UCHL1 and UCHL3 in the regulation of the central nervous system. UCHL3 functions as a de-ubiquitinating enzyme where lack of its hydrolase activity may result in the prominent accumulation of ubiquitinated proteins and subsequent induction of stress responses in skeletal muscle. UCHL3 has also been identified as a tumor-specific antigen in colon cancer.

Full Name
ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
References
  • Wood,M.A. et al., 2005, Hippocampus  15 (5):610-21.
  • Kwon,J. et al., 2006, Exp Anim  55 (1):35-43.
  • Setsuie,R. et al., 2009, Neurochem Int  54 (5-6):314-21.
  • Setsuie,R. et al., 2010, Neurochem Int  56 (8):911-8.
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