Anti-UCHL1 Magnetic Beads Immunoprecipitation (IP) Kit

Cat: MB80089-RP02
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Anti-UCHL1 Magnetic Beads-IP Kit Product Components
Components Storage
Anti-UCHL1 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
CD166 Positive Cell Lysate -20℃ for 12 months
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

[1] The IP KIT contains anti-UCHL1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-UCHL1 Magnetic Beads-IP Kit Product Description
The Anti-UCHL1 magnetic Beads, conjugated with Anti-UCHL1 antibody, are used for immuneprecipitation (IP) of UCHL1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing UCHL1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound UCHL1 proteins are dissociated from the magnetic beads using an elution buffer.
Anti-UCHL1 Magnetic Beads-IP Kit Antibody Information
Antibody
Anti-UCHL1 Antibody(80089-RP02)
Immunogen
Recombinant Rat UCHL1 protein (Catalog#80089-R07E)
Species Reactivity
Rat UCHL1
Source
Polyclonal Rat Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Rat UCHL1 (rR UCHL1; Catalog#80089-R07E; Q00981; Glu 2-Ala 223). UCHL1 specific IgG was purified by Rat UCHL1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
UCHL1 Background Information

Ubiquitin carboxyl-terminal hydrolase isozyme L1, also known as UCH-L1, Ubiquitin thioesterase L1, PGP9.5 and UCHL1, is a deubiqutinating enzyme with important functions in recycling of ubiquitin. Regulated proteolysis by the ubiquitin pathway has been implicated in control of the cell cycle, transcriptional activation, cell fate and growth, and synaptogenesis. The ubiquitin-proteasome system is involved in synaptic plasticity and is proposed to be part of a molecular switch that converts short-term synaptic potentiation to long-term changes in synaptic strength. UCHL1 is found in neuronal cell bodies and processes throughout the neocortex (at protein level). It is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. UCHL1 is weakly expressed in ovary. UCHL1 is a ubiquitin-protein hydrolase. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. UCHL1 also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. UCHL1 dysfunction has been associated with neurodegeneration in Parkinson's, Alzheimer's, and Huntington's disease patients. Reduced UCHL1 function may jeopardize the survival of CNS neurons.

Full Name
ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
References
  • Wada H., et al., 1998, Biochem. Biophys. Res. Commun. 251:688-92.
  • Choi J., et al., 2004, J. Biol. Chem. 279:13256-64.
  • Lombardino,A.2005, et al., J.Proc Natl Acad Sci.USA102 (22):8036-41
  • Okochi-Takada,E. et al., 2006, Int J Cancer. 119 (6):1338-44.
  • Zetterberg, M. et al., 2010, Mol Neurodegener  5 :11.
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