Anti-MDH1 Magnetic Beads Immunoprecipitation (IP) Kit

Cat: MB80232-RP02
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Anti-MDH1 Magnetic Beads-IP Kit Product Components
Components Storage
Anti-MDH1 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
CD166 Positive Cell Lysate -20℃ for 12 months
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

[1] The IP KIT contains anti-MDH1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-MDH1 Magnetic Beads-IP Kit Product Description
The Anti-MDH1 magnetic Beads, conjugated with Anti-MDH1 antibody, are used for immuneprecipitation (IP) of MDH1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing MDH1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound MDH1 proteins are dissociated from the magnetic beads using an elution buffer.
Anti-MDH1 Magnetic Beads-IP Kit Antibody Information
Antibody
Anti-MDH1 Antibody(80232-RP02)
Immunogen
Recombinant Rat MDHA / MDH1 protein (Catalog#80232-R08E)
Species Reactivity
Rat MDHA / MDH1
Source
Polyclonal Rat Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Rat MDHA / MDH1 (rR MDHA / MDH1; Catalog#80232-R08E; O88989; Met4-Ala334). MDHA / MDH1 specific IgG was purified by Rat MDHA / MDH1 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
MDH1 Background Information

Malate dehydrogenases 1(MDH1 / MDHA) is soluable form of malate dehydrogenases. Malate dehydrogenases (MDH) is a group of multimeric enzymes consisting of identical subunits usually organized as either dimer or tetramers with subunit molecular weights of 30-35 kDa. MDH has been isolated from different sources including archaea, eubacteria, fungi, plant and mammals. MDH catalyzes the NAD/NADH-dependent interconversion of the substrates malate and oxaloacetate. This reaction plays a key part in the malate / aspartate shuttle across the mitochondrial membrane, and in the tricarboxylic acid cycle within the mitochondrial matrix. The enzymes share a common catalytic mechanism and their kinetic properties are similar, which demonstrates a high degree of structural similarity. The three-dimensional structures and elements essential for catalysis are conserved between mitochondrial and cytoplasmic forms of MDH in eukaryotic cells even though these isoenzymes are only marginally related at the level of primary structure. 

Full Name
malate dehydrogenase 1, NAD (soluble)
References
  • Minarik P, et al. (2002) Malate dehydrogenases--structure and function. Gen Physiol Biophys. 21 (3): 257-65.
  • Musrati RA, et al. (1998) Malate dehydrogenase: distribution, function and properties. Gen Physiol Biophys. 17 (3): 193-210.
  • Hall MD, et al. (1992) Crystal structure of Escherichia coli malate dehydrogenase. A complex of the apoenzyme and citrate at 1.87 A resolution. J Mol Biol. 226 (3): 867-82.
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