Anti-IL-18 Magnetic Beads Immunoprecipitation (IP) Kit

Cat: MB90011-RP02
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Anti-IL-18 Magnetic Beads-IP Kit Product Components
Components Storage
Anti-IL-18 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
CD166 Positive Cell Lysate -20℃ for 12 months
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

[1] The IP KIT contains anti-IL-18 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

[2] Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

[3] Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

Anti-IL-18 Magnetic Beads-IP Kit Product Description
The Anti-IL-18 magnetic Beads, conjugated with Anti-IL-18 antibody, are used for immuneprecipitation (IP) of IL-18 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing IL-18 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound IL-18 proteins are dissociated from the magnetic beads using an elution buffer.
Anti-IL-18 Magnetic Beads-IP Kit Antibody Information
Antibody
Anti-IL-18 Antibody(90011-RP02)
Immunogen
Recombinant Rhesus IL18 / Interleukin-18 protein (Catalog#90011-C07E)
Species Reactivity
Cynomolgus IL18 / Interleukin-
Source
Polyclonal Rhesus Rabbit IgG
Preparation
Produced in rabbits immunized with purified, recombinant Cynomolgus IL18 / Interleukin-18 (rC IL18 / Interleukin-18; Catalog#90011-C07E; Ala 2-Asp 193). IL18 / Interleukin-18 specific IgG was purified by Cynomolgus IL18 / Interleukin-18 affinity chromatography.
Applications
Immunoprecipitation (IP), Minimum Protein Purification
IL-18 Background Information

Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1−like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cells-, IL-4− and STAT6−dependent fashion. IL-18 and T cell receptor−mediated stimulation could induce naïve CD4+ T cells to develop into IL-4−producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
interleukin 18
References
  • Dinarello CA. (1999) IL-18: A TH1-inducing, proinflammatory cytokine and new member of the IL-1 family. J Allergy Clin Immunol. 103: 11-24.
  • Takeda K, et al.. (1998) Defective NK cell activity and Th1 response in IL-18-deficient mice. Immunity. 8(3): 383-90.
  • Gracie JA, et al.. (1999) A proinflammatory role for IL-18 in rheumatoid arthritis. J Clin Invest. 104(10): 1393-401.
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