快速訂購

留言    收藏

Immunoprecipitation/ IP Kit - Anti-IGFBP-2 / IGFBP2 Immunomagnetic Beads

    產品資料評論相關產品實驗方法
    Immunoprecipitation/ IP Kit- Anti-IGFBP-2 / IGFBP2 Immunomagnetic Beads
    Product Contents
    ContentsPackage 1Package 2Storage
    Anti-IGFBP-2 / IGFBP2 Immunomagnetic Beads1 31 mL5 mL2-8℃ for 12 months
    NP40 Cell Lysis Buffer4 mL22 mL-20℃ for 12 months
    5×TBST(pH7.4)Required but not supplied
    1×TBST(pH7.4)Required but not supplied
    ddH2ORequired but not supplied
    IGFBP-2 / IGFBP2 Positive Cell Lysis300 μg300 μg-20℃ for 12 months
    Alkaline Elution Buffer3 mL15 mL2-8℃ for 12 months
    Acidity Elution Buffer3 mL15 mL2-8℃ for 12 months
    Neutralization Buffer2 mL8 mL2-8℃ for 12 months
    Magnetic Separator Not included (refer related product MAGS001)One MAGS001 included in China2

    [1] The IP KIT contains anti-IGFBP-2 / IGFBP2 Immunomagnetic Beads(2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).
    [2] The Magnetic Separator cannot be included for oversea customers due to shipment prohibition.

    [3] Shipping: Immunomagnetic Beads kits are shipped at ambient temperature in which immunomagnetic beads are provided in liquid buffer.

    Product Description

    The Anti-IGFBP-2 / IGFBP2 Immunomagnetic Beads, conjugated with Anti-IGFBP-2 / IGFBP2 antibody, are used for immuneprecipitation (IP) of IGFBP-2 / IGFBP2 proteins which expressed in vitro expression systems and bacterial and mammalian cell lysates. For IP, the beads are added to a sample containing IGFBP-2 / IGFBP2 proteins to form a bead-protein complex. The complex is removed from the solution manually using a Magnetic Separator. The bound IGFBP-2 / IGFBP2 proteins are dissociated from the Immunomagnetic Beads using an elution buffer.

    Antibody Information
    Antibody:90083-T60
    Immunogen:Recombinant Rhesus IGFBP-2 / IGFBP2 protein (Catalog#90083-C08H)
    Isotype:Rabbit IgG
    分子種屬:Rhesus
    产品或服务:1
    Clone ID:
    Molecule:IGFBP2
    抗體製備方法:Produced in rabbits immunized with purified, recombinant Cynomolgus IGFBP-2 / IGFBP2 (rh IGFBP-2 / IGFBP2; Catalog#90083-C08H; F6YKM5; Met1-Gln324). IGFBP-2 / IGFBP2 specific IgG was purified by Cynomolgus IGFBP-2 / IGFBP2 affinity chromatography.
    特異性:Rhesus IGFBP-2 / IGFBP2
    Guaranteed Applications:IP,Minimum Protein Purification
    IP Protocol

    Fig. 1 Immunoprecipitation (IP) Protocol

    The protocol (Fig. 1) uses 50 μL Anti-IGFBP-2 / IGFBP2 Immunomagnetic Beads, but this can be scaled up or down as required.
    Cell Lysis
    Cells may be lysed using any standard cell lysis protocol in accordance with your starting materials. We suggest using NP40 Cell Lysis Buffer (supplied with kit).
    Immunoprecipitate Target Antigen
    1. Add 50 μL of Immunomagnetic Beads into a 1.5 mL microcentrifuge tube.
    2. Add 150 μL of 1× TBST buffer to the Immunomagnetic Beads and gently vortex to mix.
    3. Place the tube into a Magnetic Separator to collect the beads against the wall side of the tube. Remove and discard the supernatant.
    4. Add 1 mL of 1×TBST buffer to the tube. Invert the tube several times or gently vortex to mix for 1 min. Collect Immunomagnetic Beads with a Magnetic Separator. Remove and discard the supernatant.
    5. Add the sample containing target protein (100 μg of protein in 100 μL) to the pre-washed Immunomagnetic Beads, add 400 μL of 1×TBST buffer and incubate at room temperature for 30 min with mixing.
    6. Collect the Immunomagnetic Beads with a Magnetic Separator, remove the unbounded sample and save for analysis.
    7. Add 300 μL of 5× TBST buffer to the tube and gently mix. Collect the Immunomagnetic Beads and discard the supernatant. Repeat this wash twice. 8. Add 300 μL of ddH2O to the tube and gently mix. Collect the Immunomagnetic Beads on a Magnetic Separator and discard the supernatant.
    Elute Target Antigen.
    A. Alkaline Elution Protocols
    1. Add 100 μL of Alkaline Elution buffer to the tube.
    2. Gently vortex to mix and incubate the sample at room temperature on a rotator for 5 min.
    3. Magnetically separate the Immunomagnetic Beads and save the supernatant containing the target antigen.
    4. To neutralize the sample, add 50 μL of Neutralization Buffer for each 100 μL of eluate.
    B. Acidity Elution
    1. Add 100 μL Acidity Elution Buffer.
    2. Gently vortex to mix and incubate the sample at room temperature on a rotator for 5-10 min.
    3. Magnetically separate the Immunomagnetic Beads and save the supernatant containing the target antigen.
    4. To neutralize the low pH, add 15 μL of Neutralization Buffer for each 100 μL of eluate.
    C. Elution Using Sample Buffer
    1. Add 100 μL of SDS-PAGE sample buffer to the tube.
    2. Gently vortex to mix and incubate the sample at 95-100⁰C for 5-10 min.
    3. Magnetically separate the Immunomagnetic Beads and save the supernatant containing the antigen.

    Reference Information

    Related product

    Immunoprecipitation KitCAT No.
    Magnetic Separator-1.5 (2 tubes)MAGS001
    Immunoprecipitation Kit -Immunomagnetic Beads Protein A KitBA10600
    Immunoprecipitation Kit -Immunomagnetic Beads Protein G Kit BG13103
    Immunoprecipitation Kit -Immunomagnetic Beads Protein L KitBL11044
    Immunoprecipitation Kit -Anti-DYKDDDDK(Flag®) Tag Immunomagnetic Beads Kit TB101274
    Immunoprecipitation Kit -Anti-GFP Tag Immunomagnetic Beads Kit TB13105
    Immunoprecipitation Kit -Anti-Myc Tag Immunomagnetic Beads Kit TB100029
    Immunoprecipitation Kit -Anti-HA Tag Immunomagnetic Beads Kit TB100028
    Immunoprecipitation Kit -Anti-V5 Tag Immunomagnetic Beads Kit TB100378
    Immunoprecipitation Kit -Anti-GST Tag Immunomagnetic Beads Kit TB11213

    Trouble Shooting

    ProblemPossible CauseSolution
    Little or no protein is detectedProtein degradedInclude protease inhibitors (PMSF) in the lysis buffer
    Use new lysate or lysate stored at -80℃
    No or minimal protein was expressedVerify protein expression by SDS-PAGE or Western blot
    analysis of the lysate using an positive control as a reference
    Increase the amount of lysate used for IP/Co-IP
    Use a more sensitive detection system
    Immunomagnetic Beads aggregatedImmunomagnetic beads were frozen or centrifugedHandle the Immunomagnetic Beads as directed in the instructions
    Buffer was incompatible with Immunomagnetic Beads
    Detergent was not added to the wash and bind solutions
    Failure to co-IP interacting proteinWash conditions were too stringent for the weak or transient interactionReduce the number of washes
    Lower the ionic strength of the wash buffer
    Interacting protein was expressed at a low levelApply additional protein sample
    Use a more sensitive detection system
    Buffer system was not optimal for the protein: protein interactionOptimize the co-IP buffer
    Insufficient sample was loaded on the gel for Western blot detectionElute sample in 30% acetonitrile 0.5% formic acid, then
    Bring the sample back up in SDS- PAGE Sample Buffer and load entire elution fraction on
    IGFBP-2 / IGFBP2 研究背景

    IGFBP-2, also known as IGFBP2, is a insulin-like growth factor-binding protein (IGFBP). IGFBPs prolong the half-life of the IGFs , control bioavailability, activity, and distribution of insulin-like growth factor (IGF) through high-affinity IGFBP/IGF complexes. Six high-affinity IGF-binding proteins (IGFBP-1 to -6) have been identified. The six IGFBPs are structurally related but encoded by distinct genes. IGFBPs have a high affinity for IGFs. Some members of the IGFBP family have been consistently shown to inhibit IGF actions by preventing them from gaining access to the IGF receptors, while others potentiate IGF actions by facilitating the ligand-receptor interaction. IGFBP-2 is overexpressed in many malignancies and is often correlated with an increasingly malignant status of the tumor, pointing to a potential involvement of IGFBP-2 in tumorigenesis. It contains 1 IGFBP N-terminal domain and 1 thyroglobulin type-1 domain. It inhibits IGF-mediated growth and developmental rates.

    食蟹猴 IGFBP-2 / IGFBP2 參考資料
  • Han VK, et al. (1996) The expression of insulin-like growth factor (IGF) and IGF-binding protein (IGFBP) genes in the human placenta and membranes: evidence for IGF-IGFBP interactions at the feto-maternal interface. J Clin Endocrinol Metab. 81(7): 2680-93.
  • Binkert C, et al. (1989) Cloning, sequence analysis and expression of a cDNA encoding a novel insulin-like growth factor binding protein (IGFBP-2) . EMBO J. 8(9):2497-502.
  • Wolf E, et al. (2000) Effects of IGFBP-2 overexpression in vitro and in vivo. Pediatr Nephrol. 14 (7):572-8.
  • Size / Price
    貨號: MB90083-T60-5
    目錄價: 
    單價:      (You Save: )
    規格:
    5 mL
    數量:+-
    庫存In Stock
    加入購物車大包裝查詢

    Datasheet & Documentation

    Contact Us
      您曾流覽過的產品
        All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
        請注意:所有產品都是“僅用於科研,而不能用於診斷或治療用途”